ArticleJournal of experimental botany2014
HMA4 expression in tobacco reduces Cd accumulation due to the induction of the apoplastic barrier.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Genome-wide identification and expression analysis of HMAs gene family in tobacco under abiotic stress.Scientific reports · 2026Article
- Chloroplast: structural and functional alteration under nickel stress and tolerance mechanism via the omics approach.Plant cell reports · 2026Review
- Zinc translocation from Zn-sufficient to Zn-deficient roots as an adaptation to heterogeneous Zn availability.BMC plant biology · 2025Article
- Review
- Differential absorption of cadmium and zinc by tobacco plants: Role of apoplastic pathway.Biochemistry and biophysics reports · 2024Article
- Significance and genetic control of membrane transporters to improve phytoremediation and biofortification processes.Molecular biology reports · 2023Review
- Duckweed: a potential phytosensor for heavy metals.Plant cell reports · 2022Review
- Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants.Toxics · 2022Review
- Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review.Frontiers in plant science · 2022Review
- The regulatory role of abscisic acid on cadmium uptake, accumulation and translocation in plants.Frontiers in plant science · 2022Review
- Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants.Environmental science and pollution research international · 2021Review
- Long-term effects of phytoextraction by a poplar clone on the concentration, fractionation, and transportation of heavy metals in mine tailings.Environmental science and pollution research international · 2021Article
- In situ phytoremediation of heavy metal-contaminated soil and groundwater: a green inventive approach.Environmental science and pollution research international · 2021Review
- The Role of Aquaporin Overexpression in the Modulation of Transcription of Heavy Metal Transporters under Cadmium Treatment in Poplar.Plants (Basel, Switzerland) · 2020Article
- Reduced cadmium accumulation in tobacco by sodium chloride priming.Environmental science and pollution research international · 2020Article
- Review
- Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils.Environmental science and pollution research international · 2020Review
- Expression of Genes Involved in Heavy Metal Trafficking in Plants Exposed to Salinity Stress and Elevated Cd Concentrations.Plants (Basel, Switzerland) · 2020Article
- Recent Trends in Enhancing the Resistance of Cultivated Plants to Heavy Metal Stress by Transgenesis and Transcriptional Programming.Molecular biotechnology · 2019Review
- Genotype Variation in Rice (Frontiers in plant science · 2019Article
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Authors and funding
7 authors.
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Abstract
Ectopic expression in tobacco (Nicotiana tabacum v. Xanthi) of the export protein AtHMA4 (responsible in Arabidopsis for the control of Zn/Cd root to shoot translocation) resulted in decreased Cd uptake/accumulation in roots and shoots. This study contributes to understanding the mechanisms underlying this Cd-dependent phenotype to help predict the consequences of transgene expression for potential phytoremediation/biofortification-based strategies. Microarray analysis was performed to identify metal homeostasis genes that were differentially expressed in roots of Cd-exposed AtHMA4-expressing tobacco relative to the wild type. It was established that down-regulation of genes known to mediate Cd uptake was not responsible for reduced Cd uptake/accumulation in AtHMA4 transformants. The transcript levels of NtIRT1 and NtZIP1 were higher in transgenic plants, indicating an induction of the Fe and Zn deficiency status due to AtHMA4 expression. Interestingly, upon exposure to Cd, genes involved in cell wall lignification (NtHCT, NtOMET, and NtPrx11a) were up-regulated in transformants. Microscopic analysis of roots demonstrated that expression of AtHMA4 caused an induction of cell wall lignification in the external cell layers that was accompanied by enhanced H2O2 accumulation. Further study showed that the concentration of other elements (B, Co, Cu, Ni, Mo, and Zn) was reduced in AtHMA4 transformants in the presence of Cd. In conclusion, due to ectopic expression of 35S::AtHMA4, the physical apoplastic barrier within the external cell layer developed, which is likely to be responsible for the reduction of Cd uptake/accumulation.
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