Evidence map›Paper›PMID 24419616›Full record

ArticleActa crystallographica. Section F, Structural biology communications2014

Purification, crystallization and preliminary X-ray diffraction analysis of a novel keto-deoxy-D-galactarate (KDG) dehydratase from Agrobacterium tumefaciens.

Helena Taberman, Martina Andberg, Tarja Parkkinen, Peter Richard, Nina Hakulinen, Anu Koivula, Juha Rouvinen

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Helena TabermanDepartment of Chemistry, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
Martina AndbergVTT Technical Research Centre of Finland, PO Box 1000, 02044 VTT, Finland.
Tarja ParkkinenDepartment of Chemistry, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
Peter RichardVTT Technical Research Centre of Finland, PO Box 1000, 02044 VTT, Finland.
Nina HakulinenDepartment of Chemistry, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
Anu KoivulaVTT Technical Research Centre of Finland, PO Box 1000, 02044 VTT, Finland.
Juha RouvinenDepartment of Chemistry, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
University of Eastern Finland · FIVTT Technical Research Centre of Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

D-galacturonic acid is the main component of pectin. It could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. Keto-deoxy-D-galactarate (KDG) dehydratase is an enzyme in the oxidative pathway of D-galacturonic acid in Agrobacterium tumefaciens (At). It converts 3-deoxy-2-keto-L-threo-hexarate to α-ketoglutaric semialdehyde. At KDG dehydratase was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 169.1, b = 117.8, c = 74.3 Å, β = 112.4° and an asymmetric unit of four monomers. X-ray diffraction data were collected to 1.9 Å resolution using synchrotron radiation. The three-dimensional structure of At KDG dehydratase will provide valuable information on the function of the enzyme and will allow it to be engineered for biorefinery-based applications.

Indexed as

X-Ray DiffractionAgrobacterium tumefaciensBacterial ProteinsCrystallizationElectrophoresis, Polyacrylamide GelHydro-LyasesBacterial ProteinsHydro-LyasesAgrobacterium tumefaciensd-galacturonic acidketo-deoxy-d-galactarate dehydrataseoxidative pathway

Identifiers

PMID24419616
PMCPMC3943101
OpenAlexW2037793773

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.