ReviewActa crystallographica. Section F, Structural biology communications2014
Introduction to protein crystallization.
Review in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers.
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Who cites it
142 citing papers in PubMed.
- Article
- GAMT-GINE: A Graph Isomorphism Network Integrating Continuous Spatial Awareness and Multi-Task Learning for Protein-Ligand Binding Affinity Prediction.International journal of molecular sciences · 2026Article
- Recent Advances in Particle Design for High-Concentration Protein Suspension Injectables.Pharmaceutics · 2026Review
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Article
- Crystal phase formation in crowded lysozyme solutions.RSC advances · 2026Article
- Protein Self-Assembly States Modulate Lithium Carbonate Biomineralization: From Ion Chelation to Nucleation Sites.Biomacromolecules · 2026Article
- Single mutations to tyrosine or glutamate improve the crystallizability and crystal diffraction properties of a flexible two-domain protein.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Technical Scale in Biomolecular Crystallization.Advances in biochemical engineering/biotechnology · 2026Review
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Silk Fibroin Hydrogels: Cutting-edge Developments and Future Directions.Pharmaceutical nanotechnology · 2026Review
- Flow as a Control Strategy to Promote Biomolecule Nucleation.Advances in biochemical engineering/biotechnology · 2026Review
- Biomolecular Crystallisation Through Soft Templates and Seeding.Advances in biochemical engineering/biotechnology · 2026Review
- Alternative Strategies to Promote Biomolecule Nucleation.Advances in biochemical engineering/biotechnology · 2026Review
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- The Crystallography of Enzymes: A Retrospective and Beyond.Crystals · 2025Article
- Quantifying the Influence of Poly(Ethylene glycol) on the Micelle Formation of Nonionic Detergents.ChemPlusChem · 2025Article
- Using AlphaFold and Symmetrical Docking to Predict Protein-Protein Interactions for Exploring Potential Crystallization Conditions.Proteins · 2025Article
- Assignment-Free Determination of Ligand Binding Sites in Proteins by Solid-State NMR.Journal of the American Chemical Society · 2025Article
82 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
Protein crystallization was discovered by chance about 150 years ago and was developed in the late 19th century as a powerful purification tool and as a demonstration of chemical purity. The crystallization of proteins, nucleic acids and large biological complexes, such as viruses, depends on the creation of a solution that is supersaturated in the macromolecule but exhibits conditions that do not significantly perturb its natural state. Supersaturation is produced through the addition of mild precipitating agents such as neutral salts or polymers, and by the manipulation of various parameters that include temperature, ionic strength and pH. Also important in the crystallization process are factors that can affect the structural state of the macromolecule, such as metal ions, inhibitors, cofactors or other conventional small molecules. A variety of approaches have been developed that combine the spectrum of factors that effect and promote crystallization, and among the most widely used are vapor diffusion, dialysis, batch and liquid-liquid diffusion. Successes in macromolecular crystallization have multiplied rapidly in recent years owing to the advent of practical, easy-to-use screening kits and the application of laboratory robotics. A brief review will be given here of the most popular methods, some guiding principles and an overview of current technologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.