ArticleMolecular plant pathology2014
Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Broadening Virus Resistance Through Gene Pyramiding of eIF4E Family Members.Molecular plant pathology · 2025Article
- The Naturally Occurring Amino Acid Substitution in the VPg α1-α2 Loop Breaks eIF4E-Mediated Resistance to PRSV by Enabling VPg to Re-Hijack Another eIF4E Isoform eIF(iso)4E in Watermelon.Molecular plant pathology · 2024Article
- Relationship between SugarcanePlants (Basel, Switzerland) · 2023Article
- CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Horticulture research · 2023Article
- Genome-Wide Identification and Expression Analysis of eIF Family Genes fromPlants (Basel, Switzerland) · 2022Article
- Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa.Planta · 2022Article
- Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions withThe plant pathology journal · 2021Article
- Resistance to Turnip Mosaic Virus in the FamilyThe plant pathology journal · 2021Review
- Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Plants (Basel, Switzerland) · 2020Article
- Genome Editing ofFrontiers in plant science · 2020Article
- An update on the arsenal: mining resistance genes for disease management ofHorticulture research · 2020Review
- Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses.Plant biotechnology journal · 2019Article
- A complex eIF4E locus impacts the durability of va resistance to Potato virus Y in tobacco.Molecular plant pathology · 2019Article
- Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence.Plant biotechnology journal · 2019Article
- Manipulating Cellular Factors to Combat Viruses: A Case Study From the Plant Eukaryotic Translation Initiation Factors eIF4.Frontiers in microbiology · 2019Review
- Variability in eukaryotic initiation factor iso4E in Brassica rapa influences interactions with the viral protein linked to the genome of Turnip mosaic virus.Scientific reports · 2018Article
- Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.Plant biotechnology journal · 2018Article
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- Mechanism of cytoplasmic mRNA translation.The arabidopsis book · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein-protein interaction between VPg (viral protein genome-linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad-spectrum resistance in Chinese cabbage (Brassica rapa) to Turnip mosaic virus (TuMV), which is one of the most important potyviruses, by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and B. rapa eIF(iso)4E. The seven amino acids in the cap-binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap-binding pockets, and mutated. Yeast two-hybrid assay and co-immunoprecipitation analysis demonstrated that W95L, K150L and W95L/K150E amino acid mutations of B. rapa eIF(iso)4E interrupted its interaction with TuMV VPg. All eIF(iso)4E mutants were able to complement an eIF4E-knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L, W95L/K150E and eIF(iso)4E wild-type were over-expressed in a susceptible Chinese cabbage cultivar. Evaluation of the TuMV resistance of T1 and T2 transformants demonstrated that the over-expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge-based approaches for the engineering of broad-spectrum resistance in Chinese cabbage.
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