ArticleThe journal of physical chemistry. B2014
On the potential of hyperpolarized water in biomolecular NMR studies.
Article in The journal of physical chemistry. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 78 citations in OpenAlex.
- Unmasking DNA Resonances by Suppression of Hyperpolarized Water.Journal of the American Chemical Society · 2026Article
- On the effects of hyperpolarized water-based dissolution on the solute and solventPhysical chemistry chemical physics : PCCP · 2026Article
- Residue-Specific Signatures of Structural Water Identified by Dissolution Dynamic Nuclear Polarization with UV-Generated Radicals.Angewandte Chemie (International ed. in English) · 2026Article
- The Core-Shell Conformational Space of Compartmentalized Single-Chain Nanoparticles by Paramagnetic and Hyperpolarized NMR Spectroscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Hyperpolarized NMR Reveals Low-Populated Folding Intermediates in DNA.Journal of the American Chemical Society · 2025Article
- Deep Learning for Hyperpolarized NMR of Intrinsically Disordered Proteins without Resolution Loss: Access to Short-Lived Intermediates.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Residue-Resolved Liquid-State Hyperpolarized NMR of Peptide Condensate Surfaces.Journal of the American Chemical Society · 2025Article
- Unconventional Parahydrogen-Induced Hyperpolarization Effects in Chemistry and Catalysis: From Photoreactions to Enzymes.ACS catalysis · 2025Review
- Cross-Polarization of Insensitive Nuclei from Water Protons for Detection of Protein-Ligand Binding.Journal of the American Chemical Society · 2024Article
- Cryogenic and Dissolution DNP NMR on γ-Irradiated Organic Molecules.Journal of the American Chemical Society · 2024Article
- Water-Compatible and Recyclable Heterogeneous SABRE Catalyst for NMR Signal Amplification.JACS Au · 2023Article
- Frémy's Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging.Journal of the American Chemical Society · 2022Article
- Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces.Science advances · 2022Article
- Hyperpolarized water as universal sensitivity booster in biomolecular NMR.Nature protocols · 2022Review
- Detection of Protein-Ligand Interactions byThe journal of physical chemistry letters · 2022Article
- Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water.Nature communications · 2021Article
- Parahydrogen-Induced Polarization of Amino Acids.Angewandte Chemie (International ed. in English) · 2021Review
- Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution.Communications chemistry · 2021Article
- Hyperpolarization via dissolution dynamic nuclear polarization: new technological and methodological advances.Magma (New York, N.Y.) · 2021Review
- Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR.Journal of the American Chemical Society · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A main obstacle arising when using ex situ hyperpolarization to increase the sensitivity of biomolecular NMR is the fast relaxation that macromolecular spins undergo upon being transferred from the polarizer to the spectrometer, where their observation takes place. To cope with this limitation, the present study explores the use of hyperpolarized water as a means to enhance the sensitivity of nuclei in biomolecules. Methods to achieve proton polarizations in excess of 5% in water transferred into the NMR spectrometer were devised, as were methods enabling this polarization to last for up to 30 s. Upon dissolving amino acids and polypeptides sited at the spectrometer into such hyperpolarized water, a substantial enhancement of certain biomolecular amide and amine proton resonances was observed. This exchange-driven (1)H enhancement was further passed on to side-chain and to backbone nitrogens, owing to spontaneous one-bond Overhauser processes. (15)N signal enhancements >500 over 11.7 T thermal counterparts could thus be imparted in a kinetic process that enabled multiscan signal averaging. Besides potential bioanalytical uses, this approach opens interesting possibilities in the monitoring of dynamic biomolecular processes, including solvent accessibility and exchange process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.