ArticleRedox biology2013
Novel liquid chromatography-mass spectrometry method shows that vitamin E deficiency depletes arachidonic and docosahexaenoic acids in zebrafish (Danio rerio) embryos.
Article in Redox biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Lipid Remodeling in Mouse SR-B1-Deficient Embryos with Oxidative Stress-Associated Neural Tube Defects.Antioxidants (Basel, Switzerland) · 2026Article
- Challenges of studying signaling metabolites in early development.Nature structural & molecular biology · 2026Article
- Fatty Liver in Fish: Metabolic Drivers, Molecular Pathways and Physiological Solutions.Animals : an open access journal from MDPI · 2026Review
- Deciphering the enigma of the function of alpha-tocopherol as a vitamin.Free radical biology & medicine · 2024Article
- Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges.Metabolites · 2021Review
- Vitamin E: necessary nutrient for neural development and cognitive function.The Proceedings of the Nutrition Society · 2021Article
- RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line.Antioxidants (Basel, Switzerland) · 2021Article
- A 12-lipoxygenase-Gpr31 signaling axis is required for pancreatic organogenesis in the zebrafish.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Evaluation of LC-MS and LC×LC-MS in analysis of zebrafish embryo samples for comprehensive lipid profiling.Analytical and bioanalytical chemistry · 2020Article
- Single-Cell RNA-seq Reveals Profound Alterations in Mechanosensitive Dorsal Root Ganglion Neurons with Vitamin E Deficiency.iScience · 2019Article
- Modern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples.Molecules (Basel, Switzerland) · 2019Review
- Dietary Vitamin E Status Dictates Oxidative Stress Outcomes by Modulating Effects of Fish Oil Supplementation in Alzheimer Disease Model APPMolecular neurobiology · 2018Article
- Regulation of lipid peroxidation and ferroptosis in diverse species.Genes & development · 2018Review
- Vitamin E deficiency during embryogenesis in zebrafish causes lasting metabolic and cognitive impairments despite refeeding adequate diets.Free radical biology & medicine · 2017Article
- Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization.Data in brief · 2017Article
- Lethal dysregulation of energy metabolism during embryonic vitamin E deficiency.Free radical biology & medicine · 2017Article
- Article
- Vitamin E function and requirements in relation to PUFA.The British journal of nutrition · 2015Review
- Lysophospholipid acyltransferases and eicosanoid biosynthesis in zebrafish myeloid cells.Prostaglandins & other lipid mediators · 2014Article
- Vitamin E inadequacy in humans: causes and consequences.Advances in nutrition (Bethesda, Md.) · 2014Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
To test the hypothesis that embryogenesis depends upon α-tocopherol (E) to protect embryo polyunsaturated fatty acids (PUFAs) from lipid peroxidation, new methodologies were applied to measure α-tocopherol and fatty acids in extracts from saponified zebrafish embryos. A solid phase extraction method was developed to separate the analyte classes, using a mixed mode cartridge (reverse phase, π-π bonding, strong anion exchange), then α-tocopherol and cholesterol were measured using standard techniques, while the fatty acids were quantitated using a novel, reverse phase liquid chromatography-mass spectrometry (LC-MS) approach. We also determined if α-tocopherol status alters embryonic lipid peroxidation products by analyzing 24 different oxidized products of arachidonic or docosahexaenoic (DHA) acids in embryos using LC with hybrid quadrupole-time of flight MS. Adult zebrafish were fed E- or E+ diets for 4 months, and then were spawned to obtain E- and E+ embryos. Between 24 and 72 hours post-fertilization (hpf), arachidonic acid decreased 3-times faster in E- (21 pg/h) compared with E+ embryos (7 pg/h, P<0.0001), while both α-tocopherol and DHA concentrations decreased only in E- embryos. At 36 hpf, E- embryos contained double the 5-hydroxy-eicosatetraenoic acids and 7-hydroxy-DHA concentrations, while other hydroxy-lipids remained unchanged. Vitamin E deficiency during embryogenesis depleted DHA and arachidonic acid, and increased hydroxy-fatty acids derived from these PUFA, suggesting that α-tocopherol is necessary to protect these critical fatty acids.
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Registered trials
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