ArticleCurrent ophthalmology reports2013
Role of molecular diagnostics in ocular microbiology.
Article in Current ophthalmology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Paucibacterial Microbiome and Resident DNA Virome of the Healthy Conjunctiva.Investigative ophthalmology & visual science · 2016Trial
- Review
- Mechanisms and Evolution of Antimicrobial Resistance in Ophthalmology: Surveillance, Clinical Implications, and Future Therapies.Antibiotics (Basel, Switzerland) · 2025Review
- The Ocular Surface Microbiome in Homeostasis and Dysbiosis.Microorganisms · 2025Review
- Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis.Journal of ophthalmic inflammation and infection · 2024Article
- Dual Role ofMicroorganisms · 2024Article
- Utility of pan-bacterial and pan-fungal PCR in endophthalmitis: case report and review of the literature.Journal of ophthalmic inflammation and infection · 2024Review
- Infectious Keratitis: Characterization of Microbial Diversity through Species Richness and Shannon Diversity Index.Biomolecules · 2024Article
- Pseudomonas Keratitis: From Diagnosis to Successful Deep Anterior Lamellar Keratoplasty.Cureus · 2024Article
- Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- An All-in-One Highly Multiplexed Diagnostic Assay for Rapid, Sensitive, and Comprehensive Detection of Intraocular Pathogens.American journal of ophthalmology · 2023Article
- Nonsurgical Management of Severe Viral Keratitis with Hypopyon and Retrocorneal Plaques: A Case Series.Ophthalmology and therapy · 2023Article
- Sensitive and selective colorimetric detection ofSaudi journal of biological sciences · 2023Article
- Occurrence ofMicroorganisms · 2022Article
- Deep Metagenomic Sequencing for Endophthalmitis Pathogen Detection Using a Nanopore Platform.American journal of ophthalmology · 2022Article
- Exploring the Healthy Eye Microbiota Niche in a Multicenter Study.International journal of molecular sciences · 2022Article
- Infectious keratitis: A review.Clinical & experimental ophthalmology · 2022Review
- Metagenomic Next-Generation Sequencing to Investigate Infectious Endophthalmitis of Brucella: A Case Report.Frontiers in medicine · 2022Article
- Generation and use of functionalised hydrogels that can rapidly sample infected surfaces.MethodsX · 2022Article
- Ocular Lesions in Brucella Infection: A Review of the Literature.Infection and drug resistance · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Although microbial culture remains the gold standard for diagnosis of many ocular infections, the technique is limited by low yield, inability to detect certain organisms, and potentially long delays to results. DNA-based molecular diagnostic techniques use detection of specific nucleic acid sequences as evidence for presence of suspected pathogens. The polymerase chain reaction (PCR) is a powerful molecular biology technique that allows for detection of fewer than 10 copies of pathogen genome. Recent technical advances in PCR have permitted quantitation of pathogen load using quantitative PCR (qPCR), and have permitted multiplexing of primer sets. Use of pan-bacterial and pan-fungal primers for ribosomal DNA sequences has allowed diagnosis of bacterial and fungal infections using molecular techniques. In this review, we highlight recent advances in the application of PCR to the diagnosis of anterior segment and posterior segment ocular infectious diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.