ArticlePloS one2014
A reporter assay in lamprey embryos reveals both functional conservation and elaboration of vertebrate enhancers.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Deep homology of aScience advances · 2025Article
- Sea lamprey enlightens the origin of the coupling of retinoic acid signaling to vertebrate hindbrain segmentation.Nature communications · 2024Article
- Evolution of the expression and regulation of the nuclear hormone receptor ERR gene family in the chordate lineage.Developmental biology · 2023Article
- Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Generation of knock-in lampreys by CRISPR-Cas9-mediated genome engineering.Scientific reports · 2021Article
- Programmed DNA Elimination in Vertebrates.Annual review of animal biosciences · 2021Review
- An evolutionarily ancient mechanism for regulation of hemoglobin expression in vertebrate red cells.Blood · 2020Article
- Evolutionary and Developmental Associations of Neural Crest and Placodes in the Vertebrate Head: Insights From Jawless Vertebrates.Frontiers in physiology · 2020Review
- A genome-wide assessment of the ancestral neural crest gene regulatory network.Nature communications · 2019Article
- An atlas of anterior hox gene expression in the embryonic sea lamprey head: Hox-code evolution in vertebrates.Developmental biology · 2019Article
- A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates.Nature communications · 2019Article
- I-SceI Meganuclease-mediated transgenesis in the acorn worm, Saccoglossus kowalevskii.Developmental biology · 2019Article
- Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle.Genome biology and evolution · 2016Article
- Lampreys as Diverse Model Organisms in the Genomics Era.Bioscience · 2015Article
- A Simple Predictive Enhancer Syntax for Hindbrain Patterning Is Conserved in Vertebrate Genomes.PloS one · 2015Article
- A Hox regulatory network of hindbrain segmentation is conserved to the base of vertebrates.Nature · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The sea lamprey is an important model organism for investigating the evolutionary origins of vertebrates. As more vertebrate genome sequences are obtained, evolutionary developmental biologists are becoming increasingly able to identify putative gene regulatory elements across the breadth of the vertebrate taxa. The identification of these regions makes it possible to address how changes at the genomic level have led to changes in developmental gene regulatory networks and ultimately to the evolution of morphological diversity. Comparative genomics approaches using sea lamprey have already predicted a number of such regulatory elements in the lamprey genome. Functional characterisation of these sequences and other similar elements requires efficient reporter assays in lamprey. In this report, we describe the development of a transient transgenesis method for lamprey embryos. Focusing on conserved non-coding elements (CNEs), we use this method to investigate their functional conservation across the vertebrate subphylum. We find instances of both functional conservation and lineage-specific functional evolution of CNEs across vertebrates, emphasising the utility of functionally testing homologous CNEs in their host species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.