ArticlePloS one2014
Analysis of the diversity of substrate utilisation of soil bacteria exposed to Cd and earthworm activity using generalised additive models.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Microbial degradation of organophosphorus pesticides: mechanisms, environmental impacts, and future perspectives.World journal of microbiology & biotechnology · 2026Review
- Respiration and Substrate Utilization by Marine Fungi: Their Ecological Role in an Upwelling Ecosystem.Mycobiology · 2026Article
- Lost paradise-anthropogenic pressure alters microbial functional diversity in an endangered endemic toad-habitat system.ISME communications · 2025Article
- Recover of Soil Microbial Community Functions in Beech and Turkey Oak Forests After Coppicing Interventions.Microbial ecology · 2024Article
- Detection of Changes in Soil Microbial Community Physiological Profiles in Relation to Forest Types and Presence of Antibiotics Using BIOLOG EcoPlate.Indian journal of microbiology · 2024Article
- Exploring the Interspecific Interactions and the Metabolome of the Soil Isolate Hylemonella gracilis.mSystems · 2023Article
- Metabolic Diversity of Xylariaceous Fungi Associated with Leaf Litter Decomposition.Journal of fungi (Basel, Switzerland) · 2022Article
- Phylloplane Biodiversity and Activity in the City at Different Distances from the Traffic Pollution Source.Plants (Basel, Switzerland) · 2022Article
- Microbial-assisted soil chromium immobilization through zinc and iron-enriched rice husk biochar.Frontiers in microbiology · 2022Article
- Structural and functional characteristics of soil microbial communities in response to different ecological risk levels of heavy metals.Frontiers in microbiology · 2022Article
- Macromolecular composition and substrate range of three marine fungi across major cell types.FEMS microbes · 2022Article
- Organic waste amendments for restoration of physicochemical and biological productivity of mine spoil dump for sustainable development.Environmental monitoring and assessment · 2021Article
- Spatio-temporal variations in the physiological profiles of streambed bacterial communities: implication of wastewater treatment plant effluents.World journal of microbiology & biotechnology · 2021Article
- The Biolog EcoPlate™ Technique for Assessing the Effect of Metal Oxide Nanoparticles on Freshwater Microbial Communities.Nanomaterials (Basel, Switzerland) · 2021Article
- The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains.International journal of molecular sciences · 2020Article
- Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew.Microorganisms · 2019Article
- Four years of experimental warming do not modify the interaction between subalpine shrub species.Oecologia · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biolog EcoPlates™ can be used to measure the carbon substrate utilisation patterns of microbial communities. This method results in a community-level physiological profile (CLPP), which yields a very large amount of data that may be difficult to interpret. In this work, we explore a combination of statistical techniques (particularly the use of generalised additive models [GAMs]) to improve the exploitation of CLPP data. The strength of GAMs lies in their ability to address highly non-linear relationships between the response and the set of explanatory variables. We studied the impact of earthworms (Aporrectodea caliginosa Savigny 1826) and cadmium (Cd) on the CLPP of soil bacteria. The results indicated that both Cd and earthworms modified the CLPP. GAMs were used to assess time-course changes in the diversity of substrate utilisation (DSU) using the Shannon-Wiener index. GAMs revealed significant differences for all treatments (compared to control -S-). The Cd exposed microbial community presented very high metabolic capacities on a few substrata, resulting in an initial acute decrease of DSU (i.e. intense utilization of a few carbon substrata). After 54 h, and over the next 43 h the increase of the DSU suggest that other taxa, less dominant, reached high numbers in the wells containing sources that are less suitable for the Cd-tolerant taxa. Earthworms were a much more determining factor in explaining time course changes in DSU than Cd. Accordingly, Ew and EwCd soils presented similar trends, regardless the presence of Cd. Moreover, both treatments presented similar number of bacteria and higher than Cd-treated soils. This experimental approach, based on the use of DSU and GAMs allowed for a global and statistically relevant interpretation of the changes in carbon source utilisation, highlighting the key role of earthworms on the protection of microbial communities against the Cd.
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