ArticlePloS one2014
Molecular mechanisms underlying antiproliferative and differentiating responses of hepatocarcinoma cells to subthermal electric stimulation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.
- Application of Capacitive-Resistive Electric Transfer in Physiotherapeutic Clinical Practice and Sports.International journal of environmental research and public health · 2021Pooled it
- Research Progress of Electrical Stimulation in Ischemic Heart Disease.Frontiers in cardiovascular medicine · 2021Pooled it
- Optimizing Electrical Stimulation Parameters to Enhance Viability of Dental Pulp Stem Cells in Graphene-Based Scaffolds.ACS omega · 2026Article
- An Ultrasound-Responsive Bio-Adhesive Piezoelectric Hydrogel for Osteoarthritis Cartilage.Gels (Basel, Switzerland) · 2026Article
- Bioelectronic modulation of the thymic "genetic mirror": 448 kHz radiofrequency stimulation as a novel strategy for immune tolerance induction in type 1 diabetes.Frontiers in immunology · 2026Review
- Electrical Stimulation Therapy - Dedicated to the Perfect Plastic Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Anti-Fibrotic Effects of RF Electric Currents.International journal of molecular sciences · 2023Article
- Effects of Electrical Stimulation of the Cell: Wound Healing, Cell Proliferation, Apoptosis, and Signal Transduction.Medical sciences (Basel, Switzerland) · 2023Article
- Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination.Cancers · 2022Review
- TECAR Therapy Associated with High-Intensity Laser Therapy (Hilt) and Manual Therapy in the Treatment of Muscle Disorders: A Literature Review on the Theorised Effects Supporting Their Use.Journal of clinical medicine · 2022Review
- Comparison of resistive capacitive energy transfer therapy on cadaveric molars and incisors with and without implants.Scientific reports · 2022Article
- Response of human cancer cells to simultaneous treatment with sorafenib and radiofrequency current.Oncology letters · 2021Article
- Temperature and current flow effects of different electrode placement in shoulder capacitive-resistive electric transfer applications: a cadaveric study.BMC musculoskeletal disorders · 2021Article
- Thermal and Current Flow Effects of a Capacitive-Resistive Electric Transfer Application Protocol on Chronic Elbow Tendinopathy. A Cadaveric Study.International journal of environmental research and public health · 2021Article
- Effect of Electrical Stimulation on Ocular Cells: A Means for Improving Ocular Tissue Engineering and Treatments of Eye Diseases.BioMed research international · 2021Review
- Thermal and non-thermal effects of capacitive-resistive electric transfer application on different structures of the knee: a cadaveric study.Scientific reports · 2020Article
- Thermal and non-thermal effects off capacitive-resistive electric transfer application on the Achilles tendon and musculotendinous junction of the gastrocnemius muscle: a cadaveric study.BMC musculoskeletal disorders · 2020Article
- Response of neuroblastoma cells to RF currents as a function of the signal frequency.BMC cancer · 2019Article
- Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering.Biomaterials research · 2019Review
- Liquid Metal Enabled Electrobiology: A New Frontier to Tackle Disease Challenges.Micromachines · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capacitive Resistive Electric Transfer (CRET) therapy applies currents of 0.4-0.6 MHz to treatment of inflammatory and musculoskeletal injuries. Previous studies have shown that intermittent exposure to CRET currents at subthermal doses exert cytotoxic or antiproliferative effects in human neuroblastoma or hepatocarcinoma cells, respectively. It has been proposed that such effects would be mediated by cell cycle arrest and by changes in the expression of cyclins and cyclin-dependent kinase inhibitors. The present work focuses on the study of the molecular mechanisms involved in CRET-induced cytostasis and investigates the possibility that the cellular response to the treatment extends to other phenomena, including induction of apoptosis and/or of changes in the differentiation stage of hepatocarcinoma cells. The obtained results show that the reported antiproliferative action of intermittent stimulation (5 m On/4 h Off) with 0.57 MHz, sine wave signal at a current density of 50 µA/mm(2), could be mediated by significant increase of the apoptotic rate as well as significant changes in the expression of proteins p53 and Bcl-2. The results also revealed a significantly decreased expression of alpha-fetoprotein in the treated samples, which, together with an increased concentration of albumin released into the medium by the stimulated cells, can be interpreted as evidence of a transient cytodifferentiating response elicited by the current. The fact that this type of electrical stimulation is capable of promoting both, differentiation and cell cycle arrest in human cancer cells, is of potential interest for a possible extension of the applications of CRET therapy towards the field of oncology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.