ArticlePLoS genetics2014
Single nucleus genome sequencing reveals high similarity among nuclei of an endomycorrhizal fungus.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers, 1 of them a synthesis that pooled it.
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Who cites it
105 citing papers in PubMed, 1 synthesis or guideline pooled it, 294 citations in OpenAlex.
- Exploring agro-ecological significance, knowledge gaps, and research priorities in arbuscular mycorrhizal fungi.Frontiers in microbiology · 2024Pooled it
- Transcriptomic and metabolomic analyses reveal the role of flavonoids in ectomycorrhizal symbiosis.Mycorrhiza · 2026Article
- Long-read genomics reveal extensive nuclear-specific evolution and allele-specific expression in a dikaryotic fungus.Genome research · 2025Article
- Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.Molecular plant pathology · 2025Review
- The Rhizophagus irregularis permease RiFTR1 functions without a ferroxidase partner for reductive iron transport.Scientific reports · 2025Article
- Identification of Arbuscular Mycorrhizal Fungal Isolates using MiSeq Sequencing.Microbes and environments · 2025Article
- Article
- Cross-kingdom nutrient exchange in the plant-arbuscular mycorrhizal fungus-bacterium continuum.Nature reviews. Microbiology · 2024Review
- Alternative splicing regulation in plants by SP7-like effectors from symbiotic arbuscular mycorrhizal fungi.Nature communications · 2024Article
- Detection of rare variants among nuclei populating the arbuscular mycorrhizal fungal model species Rhizophagus irregularis DAOM197198.G3 (Bethesda, Md.) · 2024Article
- Exploring the potential role of fourFrontiers in plant science · 2024Article
- Sequencing the Genomes of the First Terrestrial Fungal Lineages: What Have We Learned?Microorganisms · 2023Review
- A highly contiguous genome assembly reveals sources of genomic novelty in the symbiotic fungus Rhizophagus irregularis.G3 (Bethesda, Md.) · 2023Article
- Characterization of Arbuscular Mycorrhizal Effector Proteins.International journal of molecular sciences · 2023Review
- Stochastic nuclear organization and host-dependent allele contribution in Rhizophagus irregularis.BMC genomics · 2023Article
- Whole genome analyses based on single, field collected spores of the arbuscular mycorrhizal fungus Funneliformis geosporum.Mycorrhiza · 2022Article
- The potential role of Mucoromycotina 'fine root endophytes' in plant nitrogen nutrition.Physiologia plantarum · 2022Article
- A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica.The New phytologist · 2022Article
- A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root PathogenJournal of fungi (Basel, Switzerland) · 2022Article
- A transcriptional activator fromFrontiers in microbiology · 2022Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
22 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclei of arbuscular endomycorrhizal fungi have been described as highly diverse due to their asexual nature and absence of a single cell stage with only one nucleus. This has raised fundamental questions concerning speciation, selection and transmission of the genetic make-up to next generations. Although this concept has become textbook knowledge, it is only based on studying a few loci, including 45S rDNA. To provide a more comprehensive insight into the genetic makeup of arbuscular endomycorrhizal fungi, we applied de novo genome sequencing of individual nuclei of Rhizophagus irregularis. This revealed a surprisingly low level of polymorphism between nuclei. In contrast, within a nucleus, the 45S rDNA repeat unit turned out to be highly diverged. This finding demystifies a long-lasting hypothesis on the complex genetic makeup of arbuscular endomycorrhizal fungi. Subsequent genome assembly resulted in the first draft reference genome sequence of an arbuscular endomycorrhizal fungus. Its length is 141 Mbps, representing over 27,000 protein-coding gene models. We used the genomic sequence to reinvestigate the phylogenetic relationships of Rhizophagus irregularis with other fungal phyla. This unambiguously demonstrated that Glomeromycota are more closely related to Mucoromycotina than to its postulated sister Dikarya.
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