ArticlePLoS biology2013
Computational and genetic reduction of a cell cycle to its simplest, primordial components.
Article in PLoS biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.BMC genomics · 2024Article
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- Turing-pattern model of scaffolding proteins that establish spatial asymmetry during the cell cycle ofiScience · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- The noncoding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus.PLoS biology · 2022Article
- Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review.Biotechnology letters · 2022Review
- Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle.PLoS computational biology · 2022Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Cyclin/Forkhead-mediated coordination of cyclin waves: an autonomous oscillator rationalizing the quantitative model of Cdk control for budding yeast.NPJ systems biology and applications · 2021Review
- Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets.mSystems · 2021Article
- A bifunctional ATPase drives tad pilus extension and retraction.Science advances · 2019Article
- Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA.Nucleic acids research · 2019Article
- Absolute Measurements of mRNA Translation in Caulobacter crescentus Reveal Important Fitness Costs of Vitamin BmSystems · 2019Article
- Transposon Sequencing of Brucella abortus Uncovers Essential Genes for GrowthInfection and immunity · 2018Article
- Non-coding RNAs Potentially Controlling Cell Cycle in the ModelFrontiers in genetics · 2018Article
- Hit the right spots: cell cycle control by phosphorylated guanosines in alphaproteobacteria.Nature reviews. Microbiology · 2017Review
- Caulobacter crescentus CdnL is a non-essential RNA polymerase-binding protein whose depletion impairs normal growth and rRNA transcription.Scientific reports · 2017Article
- Article
- Article
- Growth control switch by a DNA-damage-inducible toxin-antitoxin system in Caulobacter crescentus.Nature microbiology · 2016Article
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5 authors.
Funding
Abstract
What are the minimal requirements to sustain an asymmetric cell cycle? Here we use mathematical modelling and forward genetics to reduce an asymmetric cell cycle to its simplest, primordial components. In the Alphaproteobacterium Caulobacter crescentus, cell cycle progression is believed to be controlled by a cyclical genetic circuit comprising four essential master regulators. Unexpectedly, our in silico modelling predicted that one of these regulators, GcrA, is in fact dispensable. We confirmed this experimentally, finding that ΔgcrA cells are viable, but slow-growing and elongated, with the latter mostly due to an insufficiency of a key cell division protein. Furthermore, suppressor analysis showed that another cell cycle regulator, the methyltransferase CcrM, is similarly dispensable with simultaneous gcrA/ccrM disruption ameliorating the cytokinetic and growth defect of ΔgcrA cells. Within the Alphaproteobacteria, gcrA and ccrM are consistently present or absent together, rather than either gene being present alone, suggesting that gcrA/ccrM constitutes an independent, dispensable genetic module. Together our approaches unveil the essential elements of a primordial asymmetric cell cycle that should help illuminate more complex cell cycles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.