ArticleMolecular vision2013
Pirfenidone inhibits migration, differentiation, and proliferation of human retinal pigment epithelial cells in vitro.
Article in Molecular vision, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Inhibition of TGF-β2-Induced Trabecular Meshwork Fibrosis by Pirfenidone.Translational vision science & technology · 2023Article
- Pirfenidone Attenuates the EMT Process and the Secretion of VEGF in TGF-Journal of ophthalmology · 2023Article
- Review
- Repression of Smad4 by MicroRNA-1285 moderates TGF-β-induced epithelial-mesenchymal transition in proliferative vitreoretinopathy.PloS one · 2021Article
- Pirfenidone: A novel hypothetical treatment for COVID-19.Medical hypotheses · 2020Article
- Dual Asymmetric Centrifugation Efficiently Produces a Poloxamer-Based Nanoemulsion Gel for Topical Delivery of Pirfenidone.AAPS PharmSciTech · 2020Article
- Systematic Development and Optimization of Inhalable Pirfenidone Liposomes for Non-Small Cell Lung Cancer Treatment.Pharmaceutics · 2020Article
- Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation.International journal of ophthalmology · 2019Article
- The Effects of Retinoic Acid and MAPK Inhibitors on Phosphorylation of Smad2/3 Induced by Transforming Growth Factor β1.Tuberculosis and respiratory diseases · 2019Article
- Pirfenidone inhibits epidural scar fibroblast proliferation and differentiation by regulating TGF-β1-induced Smad-dependent and -independent pathways.American journal of translational research · 2019Article
- Blocking epithelial-to-mesenchymal transition in glioblastoma with a sextet of repurposed drugs: the EIS regimen.Oncotarget · 2017Article
- Pirfenidone inhibits post-traumatic proliferative vitreoretinopathy.Eye (London, England) · 2017Article
- Pirfenidone may revert the epithelial-to-mesenchymal transition in human lung adenocarcinoma.Oncology letters · 2017Article
- Anti-fibrotic action of pirfenidone in Dupuytren's disease-derived fibroblasts.BMC musculoskeletal disorders · 2016Article
- The anti-fibrotic agent pirfenidone synergizes with cisplatin in killing tumor cells and cancer-associated fibroblasts.BMC cancer · 2016Article
- Computational Modeling Predicts Simultaneous Targeting of Fibroblasts and Epithelial Cells Is Necessary for Treatment of Pulmonary Fibrosis.Frontiers in pharmacology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the effects of pirfenidone (PFD) on the migration, differentiation, and proliferation of retinal pigment epithelial (RPE) cells and demonstrate whether the drug induces cytotoxicity.
methodsHuman RPE cells (line D407) were treated with various concentrations of PFD. Cell migration was measured with scratch assay. The protein levels of fibronectin (FN), connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), transforming growth factor beta (TGFβS), and Smads were assessed with western blot analyses. Levels of mRNA of TGFβS, FN, and Snail1 were analyzed using reverse transcriptase-polymerase chain reaction. Cell apoptosis was detected with flow cytometry using the Annexin V/PI apoptosis kit, and the percentages of cells labeled in different apoptotic stage were compared. A Trypan Blue assay was used to assess cell viability.
resultsPFD inhibited RPE cell migration. Western blot analyses showed that PFD inhibited the expression of FN, α-SMA, CTGF, TGFβ1, TGFβ2, Smad2/3, and Smad4. Similarly, PFD also downregulated mRNA levels of Snail1, FN, TGFβ1, and TGFβ2. No significant differences in cell apoptosis or viability were observed between the control and PFD-treated groups.
conclusionsPFD inhibited RPE cell migration, differentiation, and proliferation in vitro and caused no significant cytotoxicity.
Indexed as
Identifiers
24415895PMC3883732W2129492784What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.