Evidence map›Paper›PMID 24415872›Full record

ArticleWorld journal of gastroenterology2014

Apoptotic pathway induced by diallyl trisulfide in pancreatic cancer cells.

Hong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Zheng-Li Di

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
9.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 51 citations in OpenAlex.

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  11. Dietary Bioactive Diallyl Trisulfide in Cancer Prevention and Treatment.International journal of molecular sciences · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hong-Bing MaHong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Shan HuangHong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Xiao-Ran YinHong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Yang ZhangHong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Zheng-Li DiHong-Bing Ma, Shan Huang, Xiao-Ran Yin, Yang Zhang, Department of Radiotherapy of Tumor Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Second Affiliated Hospital of Xi'an Jiaotong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the effects of diallyl trisulfide (DATS), a garlic-derived organosulfur compound, in pancreatic cancer cells.

methodsHuman pancreatic cancer cells with wild-type p53 gene (Capan-2) and normal pancreatic epithelial cells (H6C7) were cultured in RPMI1640. DATS was prepared at a concentration of 100 μmol/L. Cell viability was determined via the methyl thiazolyl tetrazolium assay. Apoptotic cells were detected by TUNEL assay. Cell cycle analysis was performed using flow cytometry. Protein expression was determined by Western blot. Bax and Bcl-2 expression was detected by immunofluorescence. Apoptosis genes and cell cycle were assessed by quantitative real-time polymerase chain reaction.

resultsDATS suppressed the viability of cultured human pancreatic cancer cells (Capan-2) by increasing the proportion of cells in the G2/M phase and induced apoptotic cell death. Western blot analysis indicated that DATS enhanced the expression of Fas, p21, p53 and cyclin B1, but downregulated the expression of Akt, cyclin D1, MDM2 and Bcl-2. DATS induced cell cycle inhibition which was correlated with elevated levels of cyclin B1 and p21, and reduced levels of cyclin D1 in Capan-2 cells and H6C7 cells. DATS-induced apoptosis was markedly elevated in Capan-2 cells compared with H6C7 cells, and this was correlated with elevated levels of cyclin B1 and p53, and reduced levels of Bcl-2. DATS-induced apoptosis was correlated with down-regulation of Bcl-2, Akt and cyclin D1 protein levels, and up-regulation of Bax, Fas, p53 and cyclin B protein levels in Capan-2 cells.

conclusionDATS induces apoptosis of pancreatic cancer cells (Capan-2) and non-tumorigenic pancreatic ductal epithelial cells (H6C7).

Indexed as

Allyl CompoundsAntineoplastic Agents, PhytogenicApoptosisApoptosis Regulatory ProteinsCell Cycle ProteinsCell Line, TumorCell SurvivalDose-Response Relationship, DrugEpithelial CellsG2 Phase Cell Cycle CheckpointsHumansPancreatic DuctsPancreatic NeoplasmsSulfidesTumor Suppressor Protein p53Allyl CompoundsAntineoplastic Agents, PhytogenicApoptosis Regulatory ProteinsCell Cycle Proteinsdiallyl trisulfideSulfidesTP53 protein, humanTumor Suppressor Protein p53ApoptosisCell cycleDiallyl trisulfidep53Pancreatic cancer

Identifiers

PMID24415872
PMCPMC3886008
OpenAlexW1968393050

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.