ArticleThe Plant cell2014
The protein phosphatase RCF2 and its interacting partner NAC019 are critical for heat stress-responsive gene regulation and thermotolerance in Arabidopsis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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86 citing papers in PubMed, 173 citations in OpenAlex.
- Integrated Genomic and Functional Analyses IdentifyInternational journal of molecular sciences · 2026Article
- FaRIF as a key regulator of strawberry fruit ripening: deciphering its targets and interaction networks.Horticulture research · 2026Article
- Cloning and functional analysis of the HaNAC11 transcription factor in Haloxylon ammodendron.BMC plant biology · 2026Article
- Chromatin Topology Reconfiguration Orchestrates Thermotolerant Male Fertility via GhAL5 in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Membrane stability under heat stress: molecular signaling, lipid remodeling, and defense mechanisms in plants.Plant cell reports · 2026Review
- ThePlants (Basel, Switzerland) · 2025Article
- Heat stress triggers enhanced nuclear localization of HYPONASTIC LEAVES 1 to regulate microRNA biogenesis and thermotolerance in plants.The Plant cell · 2025Article
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- Comparative Transcriptome Analysis ofInternational journal of molecular sciences · 2025Article
- Characterization of the NAC gene family in 'Fengdan' peony (Frontiers in plant science · 2025Article
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- Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis.The Plant cell · 2024Article
- C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 promotes flowering with TAF15b by repressing the floral repressor gene FLOWERING LOCUS C.Molecules and cells · 2024Article
- Transcriptomics and metabolomics analyses of Rosa hybrida to identify heat stress response genes and metabolite pathways.BMC plant biology · 2024Article
- Genome-Wide Identification of NAC Gene Family Members of Tree Peony (International journal of molecular sciences · 2024Article
- Heat-stress-induced ROS in maize silks cause late pollen tube growth arrest and sterility.iScience · 2024Article
- Integrating physiological and multi-omics methods to elucidate heat stress tolerance for sustainable rice production.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- NACs strike again: NOR-like1 is responsible for cuticle development in tomato fruit.Journal of experimental botany · 2024Article
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- Transcriptomic and physiological analyses reveal different grape varieties response to high temperature stress.Frontiers in plant science · 2024Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat stress is a major environmental constraint for crop production worldwide. To respond to and cope with heat stress, plants synthesize heat shock proteins (HSPs), which are often molecular chaperones and are under the control of heat stress transcription factors (HSFs). Very little is known about the upstream regulators of HSFs. In a forward genetic screen for regulators of C-REPEAT BINDING FACTOR (CBF) gene expression (RCFs), we identified RCF2 and found that it is allelic to CPL1/FIERY2, which encodes a homolog of C-terminal domain phosphatase. Our results also showed that, in addition to being critical for cold stress tolerance, RCF2 is required for heat stress-responsive gene regulation and thermotolerance, because, compared with the wild type, the rcf2-1 mutant is hypersensitive to heat stress and because the reduced thermotolerance is correlated with lower expression of most of the 21 HSFs and some of the HSPs in the mutant plants. We found that RCF2 interacts with the NAC transcription factor NAC019 and that RCF2 dephosphorylates NAC019 in vivo. The nac019 mutant is more sensitive to heat stress than the wild type, and chromatin immunoprecipitation followed by quantitative PCR analysis revealed that NAC019 binds to the promoters of HSFA1b, HSFA6b, HSFA7a, and HSFC1. Overexpression of RCF2 or NAC019 in Arabidopsis thaliana increases thermotolerance. Together, our results suggest that, through dephosphorylation of NAC019, RCF2 is an integrator of high-temperature signal transduction and a mechanism for HSF and HSP activation.
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