ArticleJournal of bacteriology2014
Calvin cycle mutants of photoheterotrophic purple nonsulfur bacteria fail to grow due to an electron imbalance rather than toxic metabolite accumulation.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Purple phototrophic bacteria release crotonate as a metabolic overflow pathway.Communications biology · 2026Article
- Primary production by the purple nonsulfur bacteriumApplied and environmental microbiology · 2025Article
- Non-Respiratory Extracellular Electron Transfer Competes with Nitrogenase for Electrons in Rhodopseudomonas Palustris.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Redox poise in R. rubrum phototrophic growth drives large-scale changes in macromolecular pathways.PLoS computational biology · 2025Article
- Metabolic pathways to sustainability: review of purple non-sulfur bacteria potential in agri-food waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired.Applied and environmental microbiology · 2024Article
- Article
- Proteomic Time-Course Analysis of the Filamentous Anoxygenic Phototrophic Bacterium,Microorganisms · 2022Article
- Effects of Mixing Volatile Fatty Acids as Carbon Sources onMicroorganisms · 2021Article
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath.Applied and environmental microbiology · 2021Article
- TCA Cycle Replenishing Pathways in Photosynthetic Purple Non-Sulfur Bacteria Growing with Acetate.Life (Basel, Switzerland) · 2021Review
- Analysis of the Involvement of the Isoleucine Biosynthesis Pathway in Photoheterotrophic Metabolism ofFrontiers in microbiology · 2021Article
- Photoheterotrophic Assimilation of Valerate and Associated Polyhydroxyalkanoate Production byApplied and environmental microbiology · 2020Article
- Carbon substrate re-orders relative growth of a bacterium using Mo-, V-, or Fe-nitrogenase for nitrogen fixation.Environmental microbiology · 2020Article
- Engineering Photosynthetic Bioprocesses for Sustainable Chemical Production: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Global Proteomic Analysis Reveals High Light Intensity Adaptation Strategies and Polyhydroxyalkanoate Production inFrontiers in microbiology · 2020Article
- Influence of Energy and Electron Availability onApplied and environmental microbiology · 2019Article
- N2 gas is an effective fertilizer for bioethanol production by Zymomonas mobilis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Purple nonsulfur bacteria grow photoheterotrophically by using light for energy and organic compounds for carbon and electrons. Disrupting the activity of the CO2-fixing Calvin cycle enzyme, ribulose 1,5-bisphosphate carboxylase (RubisCO), prevents photoheterotrophic growth unless an electron acceptor is provided or if cells can dispose of electrons as H2. Such observations led to the long-standing model wherein the Calvin cycle is necessary during photoheterotrophic growth to maintain a pool of oxidized electron carriers. This model was recently challenged with an alternative model wherein disrupting RubisCO activity prevents photoheterotrophic growth due to the accumulation of toxic ribulose-1,5-bisphosphate (RuBP) (D. Wang, Y. Zhang, E. L. Pohlmann, J. Li, and G. P. Roberts, J. Bacteriol. 193:3293-3303, 2011, http://dx.doi.org/10.1128/JB.00265-11). Here, we confirm that RuBP accumulation can impede the growth of Rhodospirillum rubrum (Rs. rubrum) and Rhodopseudomonas palustris (Rp. palustris) RubisCO-deficient (ΔRubisCO) mutants under conditions where electron carrier oxidation is coupled to H2 production. However, we also demonstrate that Rs. rubrum and Rp. palustris Calvin cycle phosphoribulokinase mutants that cannot produce RuBP cannot grow photoheterotrophically on succinate unless an electron acceptor is provided or H2 production is permitted. Thus, the Calvin cycle is still needed to oxidize electron carriers even in the absence of toxic RuBP. Surprisingly, Calvin cycle mutants of Rs. rubrum, but not of Rp. palustris, grew photoheterotrophically on malate without electron acceptors or H2 production. The mechanism by which Rs. rubrum grows under these conditions remains to be elucidated.
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