ArticleJournal of bacteriology2014
An archaeal glutamate decarboxylase homolog functions as an aspartate decarboxylase and is involved in β-alanine and coenzyme A biosynthesis.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Discovery of Bacillamide-Acylhydrazone Hybrids as Novel Fungicide Lead Compounds.Journal of fungi (Basel, Switzerland) · 2026Article
- Synthesis of versatile neuromodulatory molecules by a gut microbial glutamate decarboxylase.iScience · 2025Article
- Navigating the archaeal frontier: insights and projections from bioinformatic pipelines.Frontiers in microbiology · 2024Article
- Biosynthesis of Gamma-Aminobutyric Acid (GABA) byCurrent issues in molecular biology · 2023Review
- MpADC, an L-aspartate-α-decarboxylase, from Myzus persicae, that enables production of β-alanine with high yield by whole-cell enzymatic catalysis.Biotechnology for biofuels and bioproducts · 2023Article
- How an overlooked gene in coenzyme a synthesis solved an enzyme mechanism predicament.Molecular microbiology · 2023Review
- Large-scale genetic characterization of the model sulfate-reducing bacterium,Frontiers in microbiology · 2023Article
- Recent Advances in the Siderophore Biology ofFrontiers in microbiology · 2022Review
- Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine.Bioresources and bioprocessing · 2021Article
- Article
- Potential Roles for Gamma-Aminobutyric Acid Signaling in Bacterial Communities.Bioelectricity · 2021Review
- Advances in biotechnological production of β-alanine.World journal of microbiology & biotechnology · 2021Review
- A Structurally Novel Lipoyl Synthase in the Hyperthermophilic Archaeon Thermococcus kodakarensis.Applied and environmental microbiology · 2020Article
- Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System.Inflammation · 2020Article
- Enhanced production of β-alanine through co-expressing two different subtypes of L-aspartate-α-decarboxylase.Journal of industrial microbiology & biotechnology · 2020Article
- A novel way to synthesize pantothenate in bacteria involves β-alanine synthase present in uracil degradation pathway.MicrobiologyOpen · 2020Article
- Promiscuous Enzymes Cause Biosynthesis of Diverse Siderophores in Shewanella oneidensis.Applied and environmental microbiology · 2020Article
- Protein Engineering of a Pyridoxal-5'-Phosphate-Dependent l-Aspartate-α-Decarboxylase fromMolecules (Basel, Switzerland) · 2020Article
- Utilization of Phenol as Carbon Source by the Thermoacidophilic ArchaeonFrontiers in microbiology · 2020Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
β-Alanine is a precursor for coenzyme A (CoA) biosynthesis and is a substrate for the bacterial/eukaryotic pantothenate synthetase and archaeal phosphopantothenate synthetase. β-Alanine is synthesized through various enzymes/pathways in bacteria and eukaryotes, including the direct decarboxylation of Asp by aspartate 1-decarboxylase (ADC), the degradation of pyrimidine, or the oxidation of polyamines. However, in most archaea, homologs of these enzymes are not present; thus, the mechanisms of β-alanine biosynthesis remain unclear. Here, we performed a biochemical and genetic study on a glutamate decarboxylase (GAD) homolog encoded by TK1814 from the hyperthermophilic archaeon Thermococcus kodakarensis. GADs are distributed in all three domains of life, generally catalyzing the decarboxylation of Glu to γ-aminobutyrate (GABA). The recombinant TK1814 protein displayed not only GAD activity but also ADC activity using pyridoxal 5'-phosphate as a cofactor. Kinetic studies revealed that the TK1814 protein prefers Asp as its substrate rather than Glu, with nearly a 20-fold difference in catalytic efficiency. Gene disruption of TK1814 resulted in a strain that could not grow in standard medium. Addition of β-alanine, 4'-phosphopantothenate, or CoA complemented the growth defect, whereas GABA could not. Our results provide genetic evidence that TK1814 functions as an ADC in T. kodakarensis, providing the β-alanine necessary for CoA biosynthesis. The results also suggest that the GAD activity of TK1814 is not necessary for growth, at least under the conditions applied in this study. TK1814 homologs are distributed in a wide range of archaea and may be responsible for β-alanine biosynthesis in these organisms.
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