ArticleMolecular biology reports2014
A sex-associated sequence identified by RAPD screening in gynogenetic individuals of turbot (Scophthalmus maximus).
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 50 citations in OpenAlex.
- Sex-Specific Marker Development and Identification of an XY Sex Determination System in the Tire Track Eel (Animals : an open access journal from MDPI · 2026Article
- Artificial Induction of Meiotic Gynogenesis inAnimals : an open access journal from MDPI · 2025Article
- Exploitation and Application of a New Genetic Sex Marker Based on Intron Insertion Variation of erc2 Gene in Oplegnathus punctatus.Marine biotechnology (New York, N.Y.) · 2024Article
- Characterization of the sex determining region and development of a molecular sex identification method in a Salangid fish.BMC genomics · 2024Article
- Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus.Science China. Life sciences · 2024Article
- Identification of Sex-Specific Markers and Candidate Genes Using WGS Sequencing Reveals a ZW-Type Sex-Determination System in the Chinese Soft-Shell Turtle (International journal of molecular sciences · 2024Article
- Review
- Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (International journal of molecular sciences · 2022Article
- Sex determination mechanisms and sex control approaches in aquaculture animals.Science China. Life sciences · 2022Review
- Exploration of an XX/XY Sex Determination System and Development of PCR-Based Sex-specific Markers inFrontiers in genetics · 2022Article
- Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius).BMC genomics · 2021Article
- QTL Fine Mapping for Sex Determination Region in Bighead Carp (Hypophthalmichthys nobilis) and Comparison with Silver Carp (Hypophthalmichthys molitrix).Marine biotechnology (New York, N.Y.) · 2020Article
- Article
- Satellitome landscape analysis of Megaleporinus macrocephalus (Teleostei, Anostomidae) reveals intense accumulation of satellite sequences on the heteromorphic sex chromosome.Scientific reports · 2019Article
- Construction of a Genomic Bacterial Artificial Chromosome (BAC) Library for the Prawn Macrobrachium rosenbergii and Initial Analysis of ZW Chromosome-Derived BAC Inserts.Marine biotechnology (New York, N.Y.) · 2019Article
- Article
- Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix).DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Identification of Sex-determining Loci in Pacific White Shrimp Litopeneaus vannamei Using Linkage and Association Analysis.Marine biotechnology (New York, N.Y.) · 2017Article
- Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis.Marine biotechnology (New York, N.Y.) · 2015Article
- A transcriptome derived female-specific marker from the invasive Western mosquitofish (Gambusia affinis).PloS one · 2015Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the genetic basis of sex determination mechanisms is essential for improving the productivity of farmed aquaculture fish species like turbot (Scophthalmus maximus). In culture conditions turbot males grow slower than females starting from eight months post-hatch, and this differential growth rate is maintained until sexual maturation is reached, being mature females almost twice as big as males of the same age. The goal of this study was to identify sex-specific DNA markers in turbot using comparative random amplified polymorphism DNA (RAPD) profiles in males and females to get new insights of the genetic architecture related to sex determination. In order to do this, we analyzed 540 commercial 10-mer RAPD primers in male and female pools of a gynogenetic family because of its higher inbreeding, which facilitates the detection of associations across the genome. Two sex-linked RAPD markers were identified in the female pool and one in the male pool. After the analysis of the three markers on individual samples of each pool and also in unrelated individuals, only one RAPD showed significant association with females. This marker was isolated, cloned and sequenced, containing two sequences, a microsatellite (SEX01) and a minisatellite (SEX02), which were mapped in the turbot reference map. From this map position, through a comparative mapping approach, we identified Foxl2, a relevant gene related to initial steps of sex differentiation, and Wnt4, a gene related with ovarian development, close to the microsatellite and minisatellite markers, respectively. The position of Foxl2 and Wnt4 was confirmed by linkage mapping in the reference turbot map.
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