ArticleJournal of industrial microbiology & biotechnology2014
Two promising alkaline β-glucosidases isolated by functional metagenomics from agricultural soil, including one showing high tolerance towards harsh detergents, oxidants and glucose.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- EPIC: A Machine-Learning Framework for Product-Dependent Behavior in β‑Glucosidases.ACS omega · 2026Article
- Characterization of Recombinant GH 43 β-Xylosidases and a GH 3 β-Glucosidase fromJournal of microbiology and biotechnology · 2026Article
- Characterization of β-glucosidase activity of a Lactiplantibacillus plantarum 6-phospho-β-glucosidase.Applied microbiology and biotechnology · 2025Article
- A novel GH3-β-glucosidase from soda lake metagenomic libraries with desirable properties for biomass degradation.Scientific reports · 2024Article
- Biochemical andHeliyon · 2024Article
- Polyextremophile engineering: a review of organisms that push the limits of life.Frontiers in microbiology · 2024Review
- Recent Advances in Function-based Metagenomic Screening.Genomics, proteomics & bioinformatics · 2018Review
- Cellulases from Thermophiles Found by Metagenomics.Microorganisms · 2018Review
- Increased enzymatic hydrolysis of sugarcane bagasse by a novel glucose- and xylose-stimulated β-glucosidase from Anoxybacillus flavithermus subsp. yunnanensis E13BMC biochemistry · 2017Article
- Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent.Biotechnology reports (Amsterdam, Netherlands) · 2016Article
- Molecular Detection and Environment-Specific Diversity of Glycosyl Hydrolase Family 1 β-Glucosidase in Different Habitats.Frontiers in microbiology · 2016Article
- Destructuring plant biomass: focus on fungal and extremophilic cell wall hydrolases.Plant science : an international journal of experimental plant biology · 2015Review
- Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome.Frontiers in microbiology · 2015Article
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Authors and funding
4 authors.
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Abstract
New β-glucosidase activities were identified by screening metagenomic libraries constructed with DNA isolated from the topsoil of a winter wheat field. Two of the corresponding proteins, displaying an unusual preference for alkaline conditions, were selected for purification by Ni-NTA chromatography. AS-Esc6, a 762-amino-acid enzyme belonging to glycoside hydrolase family 3, proved to be a mesophilic aryl-β-glucosidase with maximal activity around pH 8 and 40 °C. A similar pH optimum was found for AS-Esc10, a 475-amino-acid GH1-family enzyme, but this enzyme remained significantly active across a wider pH range and was also markedly more stable than AS-Esc6 at pH greater than 10. AS-Esc10 was found to degrade cellobiose and diverse aryl glycosides, with an optimal temperature of 60 °C and good stability up to 50 °C. Unlike AS-Esc6, which showed a classically low inhibitory constant for glucose (14 mM), AS-Esc10 showed enhanced activity in the presence of molar concentrations of glucose. AS-Esc10 was highly tolerant to hydrogen peroxide and also to sodium dodecyl sulfate, this being indicative of kinetic stability. This unique combination of properties makes AS-Esc10 a particularly promising candidate whose potential in biotechnological applications is worth exploring further.
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