ArticlePhotosynthesis research2014
Localization of enzymes relating to C4 organic acid metabolisms in the marine diatom, Thalassiosira pseudonana.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
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- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Growth-stage-related shifts in diatom endometabolome composition set the stage for bacterial heterotrophy.ISME communications · 2022Article
- Editorial: Metabolic Regulation of Diatoms and Other Chromalveolates.Frontiers in plant science · 2022Article
- Carbon Dioxide Concentration Mechanisms in Natural Populations of Marine Diatoms: Insights FromFrontiers in plant science · 2021Article
- Insights on the Functions and Ecophysiological Relevance of the Diverse Carbonic Anhydrases in Microalgae.International journal of molecular sciences · 2020Review
- Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.The Plant cell · 2020Review
- The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2018Article
- Responses of the marine diatom Thalassiosira pseudonana to changes in COScientific reports · 2017Article
- Discovery of PPi-type Phosphoenolpyruvate Carboxykinase Genes in Eukaryotes and Bacteria.The Journal of biological chemistry · 2015Article
- Designer diatom episomes delivered by bacterial conjugation.Nature communications · 2015Article
- CCM8: the eighth international symposium on inorganic carbon uptake by aquatic photosynthetic organisms.Photosynthesis research · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
In the genome of the marine diatom-Thalassiosira pseudonana, there are several putative genes encoding enzymes potentially constitute a classical C4 type biochemical CO2-concentrating mechanism. Two genes encode a carboxylation enzyme phosphoenolpyruvate carboxylase (PEPC)1 and PEPC2; and another two encode decarboxylation enzymes, NAD(+)-dependent malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PEPCK). These genes were tagged by the enhanced-green fluorescence protein, egfp, ligated in the transformation vector, and transformed into the cells of T. pseudonana for localization of GFP fusion products. The PEPC1:GFP fusion was localized at the matrix of the periplastidal compartment, while the PEPC2:GFP fusion was localized at the mitochondria. The NAD-ME:GFP fusion was localized in the cytosol and the PEPCK:GFP fusion at the mitochondria. The transcripts level of NAD-ME was extremely low, and PEPCK transcript was significantly induced under the dark, suggesting that PEPCK is involved in the dark metabolism such as respiration and amino acid metabolism in the mitochondria. Treatments of low-CO2grown T. pseudonana cells with inhibitors for PEPCK and PEPC efficiently dissipated the maximum rate of photosynthesis while these treatments did not affect high-affinity photosynthesis. These data strongly suggest that classical C4 enzymes play little role in the CCM in T. pseudonana.
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