ArticlePhotosynthesis research2014
Localization of putative carbonic anhydrases in the marine diatom, Thalassiosira pseudonana.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 106 citations in OpenAlex.
- Engineering and Biological Mechanisms of Microalgal COMicroorganisms · 2026Review
- Comprehensive identification and subcellular localization prediction of carbonic anhydrases in the marine haptophyte Tisochrysis lutea based on a refined genome annotation.Archives of microbiology · 2026Article
- Introduction to Carbonic Anhydrase.Sub-cellular biochemistry · 2026Review
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Periplasmic carbonic anhydrase CAH1 contributes to high inorganic carbon affinity in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Dramatic changes in mitochondrial subcellular location and morphology accompany activation of the COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.Plant physiology · 2024Article
- Dramatic Changes in Mitochondrial Subcellular Location and Morphology Accompany Activation of the CObioRxiv : the preprint server for biology · 2024Article
- Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton.Nature communications · 2023Article
- Localization and characterization θ carbonic anhydrases in Thalassiosira pseudonana.Photosynthesis research · 2023Article
- α-CAs from Photosynthetic Organisms.International journal of molecular sciences · 2022Review
- Emerging trends in environmental and industrial applications of marine carbonic anhydrase: a review.Bioprocess and biosystems engineering · 2022Review
- Biogeochemical feedbacks to ocean acidification in a cohesive photosynthetic sediment.Scientific reports · 2021Article
- Diploid genomic architecture of Nitzschia inconspicua, an elite biomass production diatom.Scientific reports · 2021Article
- The Role of Extracellular Carbonic Anhydrase in Biogeochemical Cycling: Recent Advances and Climate Change Responses.International journal of molecular sciences · 2021Review
- Characterization of a COMarine biotechnology (New York, N.Y.) · 2021Article
- Review
- Carbon Dioxide Concentration Mechanisms in Natural Populations of Marine Diatoms: Insights FromFrontiers in plant science · 2021Article
- Article
- Use of an immobilised thermostableJournal of enzyme inhibition and medicinal chemistry · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thirteen putative carbonic anhydrase (CA) genes have been identified in the marine multipolar centric diatom, Thalassiosira pseudonana, and two of these CAs have been localized previously. The first, an alpha CA (TpαCA1), was localized in the chloroplast stroma; the second, a zeta-type CA (TpζCA1), was localized to the periplasmic space. In the present study, cloning and localization of the remaining CAs were carried out. TpγCA2, TpγCA3, TpγCA4, TpγCA5, TpδCA1, TpδCA2, TpδCA3, and TpζCA1 were responsive to CO2 availability at the transcriptional level, being significantly reduced in cells grown at 0.4 % CO2, whereas TpαCA1, TpαCA2, TpαCA3, TpγCA1, and TpδCA4 transcript levels were constitutive with respect to CO2 concentration. Full-length cDNAs for TpγCA1, TpγCA2, TpγCA3, TpγCA4, TpδCA1, and TpδCA2 were isolated and fused with the enhanced-green fluorescent gene at their 3' termini. These GFP-fusion constructs were transformed into T. pseudonana, and the resulting GFP fusion products were localized using fluorescence microscopy. The δ-type TpδCA1 was localized on the periphery of the cell, strongly suggesting localization to the periplasmic space or the frustule. The δ-type TpδCA3 and the γ-type TpγCA2 were, respectively, localized in a periplastidal compartment and the cytosol. The δ-type TpδCA2, and the γ-types TpγCA1, 3, and 4 were localized in the mitochondria. The distribution of CAs in T. pseudonana contrasts notably with that of the raphid pennate diatom P. tricornutum, with likely consequences for CCM function including modes of CO2 acquisition, regions in which DIC is accumulated, and needs for minimizing CO2 leakage from the chloroplast.
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