ArticleMolecular biology reports2014
Cold acclimation induces freezing tolerance via antioxidative enzymes, proline metabolism and gene expression changes in two chrysanthemum species.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 77 citations in OpenAlex.
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- A Pan-Transcriptome Analysis Indicates Efficient Downregulation of the FIB Genes Plays a Critical Role in the Response of Alfalfa to Cold Stress.Plants (Basel, Switzerland) · 2022Article
- Comparative Analysis of Antioxidant Accumulation under Cold Acclimation, Deacclimation and Reacclimation in Winter Wheat.Plants (Basel, Switzerland) · 2022Article
- Discovery of cold-resistance genes in Vitis amurensis using bud-based quantitative trait locus mapping and RNA-seq.BMC genomics · 2022Article
- Integrative Comparative Assessment of Cold Acclimation in Evergreen and Deciduous Iris Species.Antioxidants (Basel, Switzerland) · 2022Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
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- Arbuscular mycorrhizal fungi enhanced rice proline metabolism under low temperature with nitric oxide involvement.Frontiers in plant science · 2022Article
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- Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress.Ecotoxicology (London, England) · 2021Article
- Cold Tolerance during the Reproductive Phase in Chickpea (Antioxidants (Basel, Switzerland) · 2021Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.Scientific reports · 2021Article
- Transcriptional reprogramming caused by cold acclimation in Meloidogyne incognita eggs.Genes & genomics · 2021Article
- The HalophyteFrontiers in plant science · 2021Article
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8 authors at 1 institution in 1 country.
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Abstract
Cold acclimation is necessary for chrysanthemum to achieve its genetically determined maximum freezing tolerance, but the underlying physiological and molecular mechanisms are unclear. The aim of this study was to discover whether changes in antioxidative enzymes, proline metabolism and frost-related gene expression induced by cold acclimation are related to freezing tolerance. Our results showed that the semi-lethal temperature (LT50) decreased from -7.3 to -23.5 °C in Chrysanthemum dichrum and -2.1 to -7.1 °C in Chrysanthemum makinoi, respectively, after cold acclimation for 21 days. The activities of SOD, CAT and APX showed a rapid and transient increase in the two chrysanthemum species after 1 day of cold acclimation, followed by a gradual increase during the subsequent days and then stabilization. qRT-PCR analysis showed that the expression levels of some isozyme genes (Mn SOD, CAT and APX) were upregulated, which was consistent with the SOD, CAT and APX activities, while others remained relatively constant (Fe SOD and Cu/Zn SOD). P5CS and PDH expression were increased under cold acclimation and the level of P5CS presented similar trends as proline content, indicating proline accumulation was via P5CS and PDH cooperation. Cold acclimation also promoted DREB, COR413 and CSD gene expression. The activities of three enzymes and gene expression were higher in C. dichrum than in C. makinoi after cold acclimation. Our data suggested that cold-inducible freezing-tolerance could be attributed to higher activity of antioxidant enzymes, and increased proline content and frost-related gene expression during different periods.
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