ArticleProceedings of the National Academy of Sciences of the United States of America1987
Induction and segregation of glial intermediate filament expression in the RT4 family of peripheral nervous system cell lines.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 36 citations in OpenAlex.
- Expression of the calcium-binding proteins MRP8 and MRP14 in monocytes is regulated by a calcium-induced suppressor mechanism.The Biochemical journal · 1994Article
- Glial-specific cAMP response of the glial fibrillary acidic protein gene cell lines.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Tissue-specific versus cell type-specific expression of the glial fibrillary acidic protein.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Isolation and characterization of s-myc, a member of the rat myc gene family.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- An epithelium-type cytoskeleton in a glial cell: astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes.The Journal of cell biology · 1989Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
We have found glial fibrillary acidic protein (GFAP), the major component of astrocyte intermediate filaments, to be expressed in cell lines of the RT4 peripheral neurotumor family. The RT4 family is a "stem-cell-like" cell line, RT4-AC, that spontaneously undergoes differentiation in culture to three derivative cell types. This process, termed cell-type conversion, results in a segregation among the derivative cell types of parental cell phenotypes that have been described as neuronal-like or glial-like. We have identified a 50-kDa GFAP-immunoreactive cytoskeletal protein and GFAP mRNA in continuous RT4-AC and RT4-D (glial-type derivative) cell lines, but not in two presumptive neuronal-type cell lines. This result suggests that GFAP gene expression is coordinately coupled with the expression of other glial properties during cell-type conversion. In addition, the RT4-AC and RT4-D sublines were found to significantly express GFAP only at high cell densities and not during logarithmic growth and to express GFAP precociously during morphological differentiation following treatment with 1 mM N6, O2'-dibutyryladenosine 3',5'-cyclic monophosphate. These observations closely reflect reports of glial filament expression in astrocyte cultures, suggesting that a common regulatory mechanism is employed by central and peripheral nervous system glia.
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