ArticleCritical care medicine2014
A better understanding of why murine models of trauma do not recapitulate the human syndrome.
Article in Critical care medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Creating an Extremely Long-lasting Neuroischemic Wound Model.JID innovations : skin science from molecules to population health · 2025Article
- Of mice and men: Laboratory murine models for recapitulating the immunosuppression of human sepsis.Frontiers in immunology · 2022Review
- The Effects of Biological Sex on Sepsis Treatments in Animal Models: A Systematic Review and a Narrative Elaboration on Sex- and Gender-Dependent Differences in Sepsis.Critical care explorations · 2021Review
- Transcriptomic responses from improved murine sepsis models can better mimic human surgical sepsis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Injury intensifies T cell mediated graft-versus-host disease in a humanized model of traumatic brain injury.Scientific reports · 2020Article
- Discordant gene responses to radiation in humans and mice and the role of hematopoietically humanized mice in the search for radiation biomarkers.Scientific reports · 2019Article
- Modelling physical resilience in ageing mice.Mechanisms of ageing and development · 2019Review
- Trauma Severity and Its Impact on Local Inflammation in Extremity Injury-Insights From a Combined Trauma Model in Pigs.Frontiers in immunology · 2019Article
- Comparison of post-traumatic changes in circulating and bone marrow leukocytes between BALB/c and CD-1 mouse strains.PloS one · 2019Article
- Mouse Injury Model of Polytrauma and Shock.Methods in molecular biology (Clifton, N.J.) · 2018Review
- The role of NIGMS P50 sponsored team science in our understanding of multiple organ failure.The journal of trauma and acute care surgery · 2017Article
- Murine Models of Sepsis and Trauma: Can We Bridge the Gap?ILAR journal · 2017Review
- The immune system's role in sepsis progression, resolution, and long-term outcome.Immunological reviews · 2016Review
- Sex-based differences in the genomic response, innate immunity, organ dysfunction, and clinical outcomes after severe blunt traumatic injury and hemorrhagic shock.The journal of trauma and acute care surgery · 2016Article
- Impact of haemorrhagic shock intensity on the dynamic of alarmins release in porcine poly-trauma animal model.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2016Article
- Patterns of gene expression among murine models of hemorrhagic shock/trauma and sepsis.Physiological genomics · 2016Article
- Sepsis-induced immune dysfunction: can immune therapies reduce mortality?The Journal of clinical investigation · 2016Review
- Insights into the Role of Chemokines, Damage-Associated Molecular Patterns, and Lymphocyte-Derived Mediators from Computational Models of Trauma-Induced Inflammation.Antioxidants & redox signaling · 2015Review
- Why do they die? Comparison of selected aspects of organ injury and dysfunction in mice surviving and dying in acute abdominal sepsis.Intensive care medicine experimental · 2015Article
- The future of murine sepsis and trauma research models.Journal of leukocyte biology · 2015Review
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17 authors.
Funding
Abstract
objectiveGenomic analyses from blood leukocytes have concluded that mouse injury poorly reflects human trauma at the leukocyte transcriptome. Concerns have focused on the modest severity of murine injury models, differences in murine compared with human age, dissimilar circulating leukocyte populations between species, and whether similar signaling pathways are involved. We sought to examine whether the transcriptomic response to severe trauma in mice could be explained by these extrinsic factors, by utilizing an increasing severity of murine trauma and shock in young and aged mice over time, and by examining the response in isolated neutrophil populations.
designPreclinical controlled in vivo laboratory study and retrospective cohort study.
settingLaboratory of Inflammation Biology and Surgical Science and multi-institution level 1 trauma centers. SUBJECTS: Six- to 10-week-old and 20- to 24-month-old C57BL/6 (B6) mice and two cohorts of 167 and 244 severely traumatized (Injury Severity Score > 15) adult (> 18 yr) patients.
interventionsMice underwent one of two severity polytrauma models of injury. Total blood leukocyte and neutrophil samples were collected. MEASUREMENTS AND MAIN
resultsFold expression changes in leukocyte and neutrophil genome-wide expression analyses between healthy and injured mice (p < 0.001) were compared with human total and enriched blood leukocyte expression analyses of severe trauma patients at 0.5, 1, 4, 7, 14, and 28 days after injury (Glue Grant trauma-related database). We found that increasing the severity of the murine trauma model only modestly improved the correlation in the transcriptomic response with humans, whereas the age of the mice did not. In addition, the genome-wide response to blood neutrophils (rather than total WBC) was also not well correlated between humans and mice. However, the expression of many individual gene families was much more strongly correlated after injury in mice and humans.
conclusionsAlthough overall transcriptomic association remained weak even after adjusting for the severity of injury, age of the animals, timing, and individual leukocyte populations, there were individual signaling pathways and ontogenies that were strongly correlated between mice and humans. These genes are involved in early inflammation and innate/adaptive immunity.
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