ArticleNucleic acids research2014
Organization of the human mitochondrial transcription initiation complex.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- FASTK post-transcriptional regulators - a 'FAST-tracK' in mitochondrial gene expression.Biochemical Society transactions · 2025Review
- Human mitochondrial RNA polymerase structures reveal transcription start site and slippage mechanism.Molecular cell · 2025Article
- Allogenic Mitochondria and Associated Organelle Complex Treatment Attenuates High-Fat Diet-Induced Adipose Tissue Remodeling.Circulation reports · 2025Article
- GLIS3: A novel transcriptional regulator of mitochondrial functions and metabolic reprogramming in postnatal kidney and polycystic kidney disease.Molecular metabolism · 2024Article
- RNA degradation in human mitochondria: the journey is not finished.Human molecular genetics · 2024Review
- Mechanisms and regulation of human mitochondrial transcription.Nature reviews. Molecular cell biology · 2024Review
- Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M.Bio-protocol · 2023Article
- A minimal motif for sequence recognition by mitochondrial transcription factor A (TFAM).Nucleic acids research · 2022Article
- Mitochondrial Biogenesis, Mitochondrial Dynamics, and Mitophagy in the Maturation of Cardiomyocytes.Cells · 2021Review
- Human Mitochondrial RNA Processing and Modifications: Overview.International journal of molecular sciences · 2021Review
- Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver.Journal of molecular cell biology · 2021Article
- Host mitochondrial transcriptome response to SARS-CoV-2 in multiple cell models and clinical samples.Scientific reports · 2021Article
- Selective Neuron Vulnerability in Common and Rare Diseases-Mitochondria in the Focus.Frontiers in molecular biosciences · 2021Review
- Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.The Journal of biological chemistry · 2020Review
- Mitochondrial genetic variation is enriched in G-quadruplex regions that stall DNA synthesis in vitro.Human molecular genetics · 2020Article
- Mechanisms of mammalian mitochondrial transcription.Protein science : a publication of the Protein Society · 2019Review
- Article
- Regulation of energy metabolism during early mammalian development: TEAD4 controls mitochondrial transcription.Development (Cambridge, England) · 2018Article
- Structural basis of mitochondrial transcription.Nature structural & molecular biology · 2018Review
- Human mitochondrial transcription factors TFAM and TFB2M work synergistically in promoter melting during transcription initiation.Nucleic acids research · 2017Article
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9 authors.
Funding
Abstract
Initiation of transcription in human mitochondria involves two factors, TFAM and TFB2M, in addition to the mitochondrial RNA polymerase, POLRMT. We have investigated the organization of the human mitochondrial transcription initiation complex on the light-strand promoter (LSP) through solution X-ray scattering, electron microscopy (EM) and biochemical studies. Our EM results demonstrate a compact organization of the initiation complex, suggesting that protein-protein interactions might help mediate initiation. We demonstrate that, in the absence of DNA, only POLRMT and TFAM form a stable interaction, albeit one with low affinity. This is consistent with the expected transient nature of the interactions necessary for initiation and implies that the promoter DNA acts as a scaffold that enables formation of the full initiation complex. Docking of known crystal structures into our EM maps results in a model for transcriptional initiation that strongly correlates with new and existing biochemical observations. Our results reveal the organization of TFAM, POLRMT and TFB2M around the LSP and represent the first structural characterization of the entire mitochondrial transcriptional initiation complex.
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