ArticleNature chemical biology2014
Designable DNA-binding domains enable construction of logic circuits in mammalian cells.
Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 108 citations in OpenAlex.
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- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- The switch-liker's guide to plant synthetic gene circuits.The Plant journal : for cell and molecular biology · 2025Review
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- Engineering signalling pathways in mammalian cells.Nature biomedical engineering · 2024Review
- The art of designed coiled-coils for the regulation of mammalian cells.Cell chemical biology · 2024Review
- Integrated compact regulators of protein activity enable control of signaling pathways and genome-editing in vivo.Cell discovery · 2024Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems.Seminars in cell & developmental biology · 2023Review
- Construction of the genetic switches in response to mannitol based on artificial MtlR box.Bioresources and bioprocessing · 2023Article
- Synthetic memory circuits for stable cell reprogramming in plants.Nature biotechnology · 2022Article
- PERSIST platform provides programmable RNA regulation using CRISPR endoRNases.Nature communications · 2022Article
- Programmable allosteric DNA regulations for molecular networks and nanomachines.Science advances · 2022Article
- Building the SynBio community in the Czech Republic from the bottom up: You get what you give.Biotechnology notes (Amsterdam, Netherlands) · 2022Article
- Control of mammalian cell-based devices with genetic programming.Current opinion in systems biology · 2021Article
- Plant cell cultures as heterologous bio-factories for secondary metabolite production.Plant communications · 2021Review
- Context-aware synthetic biology by controller design: Engineering the mammalian cell.Cell systems · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electronic computer circuits consisting of a large number of connected logic gates of the same type, such as NOR, can be easily fabricated and can implement any logic function. In contrast, designed genetic circuits must employ orthogonal information mediators owing to free diffusion within the cell. Combinatorial diversity and orthogonality can be provided by designable DNA- binding domains. Here, we employed the transcription activator-like repressors to optimize the construction of orthogonal functionally complete NOR gates to construct logic circuits. We used transient transfection to implement all 16 two-input logic functions from combinations of the same type of NOR gates within mammalian cells. Additionally, we present a genetic logic circuit where one input is used to select between an AND and OR function to process the data input using the same circuit. This demonstrates the potential of designable modular transcription factors for the construction of complex biological information-processing devices.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.