ArticleScientific reports2014
Promotion of quality standard of herbal medicine by constituent removing and adding.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
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- Can Nature Overcome Invasive Gastrointestinal Infections?International journal of molecular sciences · 2025Review
- The extract of an herbal medicineFrontiers in pharmacology · 2024Article
- Targeting gut microbiota and immune crosstalk: potential mechanisms of natural products in the treatment of atherosclerosis.Frontiers in pharmacology · 2023Review
- Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses.Acta pharmaceutica Sinica. B · 2022Review
- Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection.Journal of food and drug analysis · 2019Article
- UPLC-QTOF/MSFrontiers in pharmacology · 2018Article
- A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study.Scientific reports · 2017Article
- Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.Scientific reports · 2017Article
- A New Strategy for Quality Evaluation and Identification of Representative Chemical Components inEvidence-based complementary and alternative medicine : eCAM · 2017Article
- Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections.Scientific reports · 2016Article
- Discovery and Current Status of Evaluation System of Bioavailability and Related Pharmaceutical Technologies for Traditional Chinese Medicines--Flos Lonicerae Japonicae--Fructus Forsythiae Herb Couples as an Example.International journal of molecular sciences · 2015Review
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify major active constituents and measure their levels in a typical medicinal herb-Rhizoma coptidis, we applied the concept of removing and adding, taking inspiration from functional genetic methods. As this herb has bacteriostatic properties and is used to treat bacterial diarrhea, we examined the effects of individual constituents (berberine, palmatine, coptisine, epiberberine, jateorrhizine and columbamine) on the growth of Shigella dysenteriae with microcalorimetry. The removing and adding procedures revealed that berberine and coptisine were the main antibacterial constituents of R. coptidis, with bacteriostatic activities of 54.10% and 39.75%, respectively. The relative levels of berberine and coptisine in R. coptidis were 8.08%-31.92% and 4.05%-14.45%, respectively. On the basis of whole effect, the method of constituents removing and adding, coupled with a bioassay, is a useful strategy to identify the active constituents and measure their levels in herbal medicines, which may provide reference to other natural products.
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