ArticleCell reports2014
The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.
Article in Cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Antibiotic-producing microorganisms: taxonomic diversity, screening strategies, and emerging and underexploited antibacterial targets.Archives of microbiology · 2026Review
- Dehydroamino acid residues in bioactive natural products.Natural product reports · 2024Review
- Hyper-swivel head domain motions are required for complete mRNA-tRNA translocation and ribosome resetting.Nucleic acids research · 2022Article
- Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch.Nature communications · 2021Article
- Development and Challenges of Antimicrobial Peptides for Therapeutic Applications.Antibiotics (Basel, Switzerland) · 2020Review
- Network theory of the bacterial ribosome.PloS one · 2020Article
- Structural basis for ribosome recycling by RRF and tRNA.Nature structural & molecular biology · 2020Article
- Article
- Draft Genome Sequence ofMicrobiology resource announcements · 2019Article
- Translational control of antibiotic resistance.Open biology · 2019Review
- From Worms to Drug Candidate: The Story of Odilorhabdins, a New Class of Antimicrobial Agents.Frontiers in microbiology · 2019Review
- Ribosome-Targeting Antibiotics: Modes of Action, Mechanisms of Resistance, and Implications for Drug Design.Annual review of biochemistry · 2018Review
- High-Quality Draft Genome Sequence of Sphaerisporangium cinnabarinum ATCC 31213.Genome announcements · 2018Article
- Exploring allosteric communication in multiple states of the bacterial ribosome using residue network analysis.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- The Mechanisms of Action of Ribosome-Targeting Peptide Antibiotics.Frontiers in molecular biosciences · 2018Review
- Similarity and diversity of translational GTPase factors EF-G, EF4, and BipA: From structure to function.RNA biology · 2016Review
- Bacterial Protein Synthesis as a Target for Antibiotic Inhibition.Cold Spring Harbor perspectives in medicine · 2016Review
- Structures of proline-rich peptides bound to the ribosome reveal a common mechanism of protein synthesis inhibition.Nucleic acids research · 2016Article
- Antimicrobial Drugs in Fighting against Antimicrobial Resistance.Frontiers in microbiology · 2016Review
- Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch.Structure (London, England : 1993) · 2015Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The translocation of mRNA and tRNA through the ribosome is catalyzed by elongation factor G (EF-G), a universally conserved guanosine triphosphate hydrolase (GTPase). The mechanism by which the closely related decapeptide antibiotics dityromycin and GE82832 inhibit EF-G-catalyzed translocation is elucidated in this study. Using crystallographic and biochemical experiments, we demonstrate that these antibiotics bind to ribosomal protein S12 in solution alone as well as within the small ribosomal subunit, inducing long-range effects on the ribosomal head. The crystal structure of the antibiotic in complex with the 70S ribosome reveals that the binding involves conserved amino acid residues of S12 whose mutations result in in vitro and in vivo antibiotic resistance and loss of antibiotic binding. The data also suggest that GE82832/dityromycin inhibits EF-G-catalyzed translocation by disrupting a critical contact between EF-G and S12 that is required to stabilize the posttranslocational conformation of EF-G, thereby preventing the ribosome-EF-G complex from entering a conformation productive for translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.