ReviewMethods (San Diego, Calif.)2014
Targeted genome engineering techniques in Drosophila.
Review in Methods (San Diego, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Adipocyte-DerivedBiomolecules · 2025Article
- Versatile Endogenous Editing of GluRIIA inCells · 2024Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- The Use ofBiomedicines · 2022Review
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
- Loss of Pseudouridine Synthases in the RluA Family Causes Hypersensitive Nociception inG3 (Bethesda, Md.) · 2020Article
- CRISPR/Cas9-mediated PBP1 and PBP3 mutagenesis induced significant reduction in electrophysiological response to sex pheromones in male Chilo suppressalis.Insect science · 2019Article
- The use of non-rodent model species in microbiota studies.Laboratory animals · 2019Review
- CRISPR/Cas9 mediated high efficiency knockout of the eye color gene Vermillion in Helicoverpa zea (Boddie).PloS one · 2018Article
- Engineering the Drosophila Genome for Developmental Biology.Journal of developmental biology · 2017Review
- Article
- A Short History and Description ofGenetics · 2017Article
- Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Frontiers in physiology · 2017Review
- Neural Circuits Underlying Fly Larval Locomotion.Current pharmaceutical design · 2017Review
- Drosophila tools and assays for the study of human diseases.Disease models & mechanisms · 2016Review
- FlyBase portals to human disease research using Drosophila models.Disease models & mechanisms · 2016Review
- Modeling congenital disease and inborn errors of development in Drosophila melanogaster.Disease models & mechanisms · 2016Review
- The Rise of CRISPR/Cas for Genome Editing in Stem Cells.Stem cells international · 2016Review
- Article
- Genome editing technologies: defining a path to clinic.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
For a century, Drosophila has been a favored organism for genetic research. However, the array of materials and methods available to the Drosophila worker has expanded dramatically in the last decade. The most common gene targeting tools, zinc finger nucleases, TALENs, and RNA-guided CRISPR/Cas9, have all been adapted for use in Drosophila, both for simple mutagenesis and for gene editing via homologous recombination. For each tool, there exist a number of web sites, design applications, and delivery methods. The successful application of any of these tools also requires an understanding of methods for detecting successful genome modifications. This article provides an overview of the available gene targeting tools and their application in Drosophila. In lieu of simply providing a protocol for gene targeting, we direct the researcher to resources that will allow access to the latest research in this rapidly evolving field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.