ArticleBiophysical journal2014
Geometrical membrane curvature as an allosteric regulator of membrane protein structure and function.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- The Hidden Cost of Fusion: Intrinsic Asymmetry in Vesicle Fusion Restricts Synthetic Cell Growth.bioRxiv : the preprint server for biology · 2025Article
- Membrane Proteins and Membrane Curvature: Mutual Interactions and a Perspective on Disease Treatments.Biomolecules · 2023Review
- Protein-Mediated Electroporation in a Cardiac Voltage-Sensing Domain Due to an nsPEF Stimulus.International journal of molecular sciences · 2023Article
- Understanding How Cells Probe the World: A Preliminary Step towards Modeling Cell Behavior?International journal of molecular sciences · 2023Article
- Beyond pore formation: reorganization of the plasma membrane induced by pore-forming proteins.Cellular and molecular life sciences : CMLS · 2021Review
- Exploring the Conformational Changes Induced by Nanosecond Pulsed Electric Fields on the Voltage Sensing Domain of a CaMembranes · 2021Article
- Single Cell-like Systems Reveal Active Unidirectional and Light-Controlled Transport by Nanomachineries.ACS nano · 2021Article
- A large size-selective DNA nanopore with sensing applications.Nature communications · 2019Article
- The 2018 biomembrane curvature and remodeling roadmap.Journal of physics D: Applied physics · 2018Article
- Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs.Biophysical journal · 2018Article
- Membrane Curvature Sensing by Amphipathic Helices: Insights from Implicit Membrane Modeling.Biophysical journal · 2018Article
- Single-molecule analysis of phospholipid scrambling by TMEM16F.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanical Transduction and the Dark Energy of Biology.Biophysical journal · 2018Review
- Artificial cell mimics as simplified models for the study of cell biology.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- New Continuum Approaches for Determining Protein-Induced Membrane Deformations.Biophysical journal · 2017Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- Recursive Alterations of the Relationship between Simple Membrane Geometry and Insertion of Amphiphilic Motifs.Membranes · 2017Review
- Lipid-Targeting Peptide Probes for Extracellular Vesicles.Journal of cellular physiology · 2016Article
- Continuum descriptions of membranes and their interaction with proteins: Towards chemically accurate models.Biochimica et biophysica acta · 2016Review
- Direct observation of proton pumping by a eukaryotic P-type ATPase.Science (New York, N.Y.) · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmembrane proteins are embedded in cellular membranes of varied lipid composition and geometrical curvature. Here, we studied for the first time the allosteric effect of geometrical membrane curvature on transmembrane protein structure and function. We used single-channel optical analysis of the prototypic transmembrane β-barrel α-hemolysin (α-HL) reconstituted on immobilized single small unilamellar liposomes of different diameter and therefore curvature. Our data demonstrate that physiologically abundant geometrical membrane curvatures can enforce a dramatic allosteric regulation (1000-fold inhibition) of α-HL permeability. High membrane curvatures (1/diameter ~1/40 nm(-1)) compressed the effective pore diameter of α-HL from 14.2 ± 0.8 Å to 11.4 ± 0.6 Å. This reduction in effective pore area (~40%) when combined with the area compressibility of α-HL revealed an effective membrane tension of ~50 mN/m and a curvature-imposed protein deformation energy of ~7 kBT. Such substantial energies have been shown to conformationally activate, or unfold, β-barrel and α-helical transmembrane proteins, suggesting that membrane curvature could likely regulate allosterically the structure and function of transmembrane proteins in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.