ArticleBiophysical journal2014
Actomyosin pulls to advance the nucleus in a migrating tissue cell.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Nuclear mechanobiology in confined cell migration.Nucleus (Austin, Tex.) · 2026Review
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.The Journal of cell biology · 2024Article
- The Migration and the Fate of Dental Pulp Stem Cells.Biology · 2023Review
- Plectin linkages are mechanosensitive and required for the nuclear piston mechanism of three-dimensional cell migration.Molecular biology of the cell · 2022Article
- The Nuclear Lamina.Cold Spring Harbor perspectives in biology · 2022Review
- Push or pull: how cytoskeletal crosstalk facilitates nuclear movement through 3D environments.Physical biology · 2022Review
- Wound healing of the corneal epithelium: a review.Asian biomedicine : research, reviews and news · 2021Review
- Calculation of the force field required for nucleus deformation during cell migration through constrictions.PLoS computational biology · 2021Article
- A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.Journal of cell science · 2021Article
- Article
- Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent.International journal of molecular sciences · 2020Article
- Nesprin-2 accumulates at the front of the nucleus during confined cell migration.EMBO reports · 2020Article
- Mechanical Cues Affect Migration and Invasion of Cells From Three Different Directions.Frontiers in cell and developmental biology · 2020Review
- Mechanical Regulation of Nuclear Translocation in Migratory Neurons.Frontiers in cell and developmental biology · 2020Review
- Cell and tissue morphology determine actin-dependent nuclear migration mechanisms in neuroepithelia.The Journal of cell biology · 2019Article
- Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Emerging regulators of vascular smooth muscle cell migration.Journal of muscle research and cell motility · 2019Review
- Local, transient tensile stress on the nuclear membrane causes membrane rupture.Molecular biology of the cell · 2019Article
- The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate.Journal of biological engineering · 2019Review
- Nuclear positioning in migrating fibroblasts.Seminars in cell & developmental biology · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The cytoskeletal forces involved in translocating the nucleus in a migrating tissue cell remain unresolved. Previous studies have variously implicated actomyosin-generated pushing or pulling forces on the nucleus, as well as pulling by nucleus-bound microtubule motors. We found that the nucleus in an isolated migrating cell can move forward without any trailing-edge detachment. When a new lamellipodium was triggered with photoactivation of Rac1, the nucleus moved toward the new lamellipodium. This forward motion required both nuclear-cytoskeletal linkages and myosin activity. Apical or basal actomyosin bundles were found not to translate with the nucleus. Although microtubules dampen fluctuations in nuclear position, they are not required for forward translocation of the nucleus during cell migration. Trailing-edge detachment and pulling with a microneedle produced motion and deformation of the nucleus suggestive of a mechanical coupling between the nucleus and the trailing edge. Significantly, decoupling the nucleus from the cytoskeleton with KASH overexpression greatly decreased the frequency of trailing-edge detachment. Collectively, these results explain how the nucleus is moved in a crawling fibroblast and raise the possibility that forces could be transmitted from the front to the back of the cell through the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.