ArticleAMB Express2014
Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16.
Article in AMB Express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
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- Hyaluronic Acid-Palmitate Nanoparticle Delivery of Carbonic Anhydrase Inhibitors Impairs Growth and Early Metabolism inInternational journal of molecular sciences · 2026Article
- Roles of three carbonic anhydrases inFEMS microbes · 2026Article
- Microbial metabolism in deep terrestrial subsurface communities - amino acids as biosignatures.Current research in microbial sciences · 2026Article
- Microbial signature of groundwater mixing in geothermal areas: insights from the Cimino-Vico volcanic system (central Italy).Scientific reports · 2025Article
- Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems inACS synthetic biology · 2025Article
- Physiology-informed use of Cupriavidus necator in biomanufacturing: a review of advances and challenges.Microbial cell factories · 2025Review
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalystmSphere · 2024Article
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Strain and model development for auto- and heterotrophic 2,3-butanediol production using Cupriavidus necator H16.Biotechnology for biofuels and bioproducts · 2024Article
- Polyhydroxyalkanoate Copolymer Production by RecombinantBioengineering (Basel, Switzerland) · 2024Article
- Engineering artificial photosynthesis based on rhodopsin for CONature communications · 2023Article
- Prospects for engineeringEngineering microbiology · 2023Review
- Comparative genomic insights into habitat adaptation of coral-associatedFrontiers in microbiology · 2023Article
- Trajectories for the evolution of bacterial COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from COMicrobial cell factories · 2022Article
- The reliance of glycerol utilization by Cupriavidus necator on COApplied microbiology and biotechnology · 2022Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Wide range of metabolic adaptations to the acquisition of the Calvin cycle revealed by comparison of microbial genomes.PLoS computational biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonic anhydrase (CA) enzymes catalyze the interconversion of CO2 and bicarbonate. These enzymes play important roles in cellular metabolism, CO2 transport, ion transport, and internal pH regulation. Understanding the metabolic role of CAs in the chemolithoautotropic bacterium Ralstonia eutropha is important for the development of high performance fermentation processes based on the bacterium's capability to fix carbon using the Calvin-Benson-Bassham (CBB) cycle. Analysis of the R. eutropha H16 genome sequence revealed the presence of four CA genes: can, can2, caa and cag. We evaluated the importance of each of the CAs in the metabolism of R. eutropha by examination of growth and enzyme activity in gene deletion, complementation, and overexpression strains. All four purified CAs were capable of performing the interconversion of CO2 and HCO3-, although the equilibrium towards the formation of CO2 or HCO3- differs with each CA. Deletion of can, encoding a β-CA, affected the growth of R. eutropha; however the growth defect could be compensated by adding CO2 to the culture. Deletion of the caa, encoding an α-CA, had the strongest deleterious influence on cell growth. Strains with deletion or overexpression of can2 or cag genes exhibited similar behavior to wild type under most of the conditions tested. In this work, Caa was studied in greater detail using microscopy and complementation experiments, which helped confirm its periplasmic localization and determine its importance for robust growth of R. eutropha. A hypothesis for the coordinated role of these four enzymes in the metabolism of R. eutropha is proposed.
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