ArticleAnalytical chemistry2014
Anions in electrothermal supercharging of proteins with electrospray ionization follow a reverse Hofmeister series.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Lyotropy as a Design Consideration for Ultra-Small Protein Nanoparticles via Electrohydrodynamic Jetting.Macromolecular rapid communications · 2025Article
- The Role of Nanobubbles in Protein Unfolding during Electrothermal Supercharging.Journal of the American Society for Mass Spectrometry · 2025Article
- Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry.Analytical chemistry · 2020Article
- Comparing the Effects of Additives on Protein Analysis Between Desorption Electrospray (DESI) and Electrospray Ionization (ESI).Journal of the American Society for Mass Spectrometry · 2018Article
- OH-Radical Oxidation of Lung Surfactant Protein B on Aqueous Surfaces.Mass spectrometry (Tokyo, Japan) · 2018Article
- Protein-Glass Surface Interactions and Ion Desalting in Electrospray Ionization with Submicron Emitters.Journal of the American Society for Mass Spectrometry · 2018Article
- The Effect of Salts in Promoting Specific and Competitive Interactions between Zinc Finger Proteins and Metals.Journal of the American Society for Mass Spectrometry · 2017Article
- Joule Heating and Thermal Denaturation of Proteins in Nano-ESI Theta Tips.Journal of the American Society for Mass Spectrometry · 2017Article
- Addressing a Common Misconception: Ammonium Acetate as Neutral pH "Buffer" for Native Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Ammonium Bicarbonate Addition Improves the Detection of Proteins by Desorption Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Isolating Protein Charge State Reduction in Electrospray Droplets Using Femtosecond Laser Vaporization.Journal of the American Society for Mass Spectrometry · 2017Article
- Electrothermal supercharging of proteins in native MS: effects of protein isoelectric point, buffer, and nanoESI-emitter tip size.The Analyst · 2016Article
- Real-time HD Exchange Kinetics of Proteins from Buffered Aqueous Solution with Electrothermal Supercharging and Top-Down Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- The use of chromium(III) to supercharge peptides by protonation at low basicity sites.Journal of the American Society for Mass Spectrometry · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The effects of different anions on the extent of electrothermal supercharging of proteins from aqueous ammonium and sodium salt solutions were investigated. Sulfate and hydrogen phosphate are the most effective anions at producing high charge state protein ions from buffered aqueous solution, whereas iodide and perchlorate are ineffective with electrothermal supercharging. The propensity for these anions to produce high charge state protein ions follows the following trend: sulfate > hydrogen phosphate > thiocyanate > bicarbonate > chloride > formate ≈ bromide > acetate > iodide > perchlorate. This trend correlates with the reverse Hofmeister series over a wide range of salt concentrations (1 mM to 2 M) and with several physical properties, including solvent surface tension, anion viscosity B-coefficient, and anion surface/bulk partitioning coefficient, all of which are related to the Hofmeister series. The effectiveness of electrothermal supercharging does not depend on bubble formation, either from thermal degradation of the buffer or from coalescence of dissolved gas. These results provide evidence that the effect of different ions in the formation of high charge state ions by electrothermal supercharging is largely a result of Hofmeister effects on protein stability leading to protein unfolding in the heated ESI droplet.
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