ArticleAnalytical chemistry2014
Development and quantitative evaluation of a high-resolution metabolomics technology.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
106 citing papers in PubMed, 188 citations in OpenAlex.
- Morphomics, Survival, and Metabolites in Patients With Metastatic Pancreatic Cancer.JAMA network open · 2024Trial
- Impact of Topical Interventions on the Vaginal Microbiota and Metabolome in Postmenopausal Women: A Secondary Analysis of a Randomized Clinical Trial.JAMA network open · 2022Trial
- Gut Metabolism Links Precision Nutrition, Exercise, and Healthspan in Drosophila melanogaster.Aging cell · 2026Article
- Exercise suppresses breast cancer and reprograms the immune tumor microenvironment, cancer cell intrinsic features, and their interaction.Journal of sport and health science · 2026Article
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- Analytical Methods for Mass Spectrometry-Based Metabolomics Studies.Advances in experimental medicine and biology · 2026Review
- Gut metabolism links precision nutrition, exercise, and healthspan inbioRxiv : the preprint server for biology · 2025Article
- Multi-Omics Assessment of Puff Volume-Mediated Salivary Biomarkers of Metal Exposure and Oxidative Injury Associated with Electronic Nicotine Delivery Systems.Environmental health perspectives · 2025Article
- Association of serum metabolites with frailty phenotype and its components: a cross-sectional case-control study.Biogerontology · 2024Article
- Dietary pro-oxidant therapy by a vitamin K precursor targets PI 3-kinase VPS34 function.Science (New York, N.Y.) · 2024Article
- Separation of reproductive decline from lifespan extension during methionine restriction.Nature aging · 2024Article
- Targeting glutamine metabolism improves sarcoma response to radiation therapy in vivo.Communications biology · 2024Article
- Untargeted serum metabolic profiling of diabetes mellitus among Parkinson's disease patients.NPJ Parkinson's disease · 2024Article
- Lactate Utilization Enables Metabolic Escape to Confer Resistance to BET Inhibition in Acute Myeloid Leukemia.Cancer research · 2024Article
- Article
- Regulation of urea cycle by reversible high-stoichiometry lysine succinylation.Nature metabolism · 2024Article
- GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis.Nature communications · 2023Article
- Methionine availability influences essential H3K36me3 dynamics during cell differentiation.bioRxiv : the preprint server for biology · 2023Article
- Article
- Hedgehog Signaling Regulates Treg to Th17 Conversion Through Metabolic Rewiring in Breast Cancer.Cancer immunology research · 2023Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recent advances in mass spectrometry have allowed for unprecedented characterization of human metabolism and its contribution to disease. Despite these advances, limitations in metabolomics technology remain. Here, we describe a metabolomics strategy that consolidates several recent improvements in mass spectrometry technology. The platform involves a high-resolution Orbitrap mass spectrometer coupled to faster scanning speeds, allowing for polarity switching and improved ion optics resulting in enhanced sensitivity. When coupled to HILIC chromatography, we are able to quantify over 339 metabolites from an extract of HCT8 cells with a linear range of over 4 orders of magnitude in a single chromatographic run. These metabolites include diverse chemical classes ranging from amino acids to polar lipids. In addition, we also detect over 3000 additional potential metabolites present in mammalian cells. We applied this platform to characterize the metabolome of eight colorectal cancer cell lines and observed both commonalities and heterogeneities across their metabolic profiles when cells are grown in identical conditions. Together these results demonstrate that simultaneous profiling and quantitation of the human metabolome is feasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.