ArticlePloS one2014
Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
- A Simple Method for Estimating the Spatiotemporal Distribution of Phenoloxidase Proteins in Insect Tissues.Bio-protocol · 2026Article
- Imidacloprid soil drenches indirectly weaken the selection and predatory ability of the coccinellid predator Propylea japonica (Coleoptera: Coccinellidae).Scientific reports · 2025Article
- Article
- Selection of appropriate reference genes for RT-qPCR analysis in Propylea japonica (Coleoptera: Coccinellidae).PloS one · 2018Article
- Toxicological and Biochemical Analyses Demonstrate the Absence of Lethal or Sublethal Effects ofFrontiers in plant science · 2018Article
- Bt rice in China - focusing the nontarget risk assessment.Plant biotechnology journal · 2017Article
- Article
- No impact of transgenic cry1C rice on the rove beetle Paederus fuscipes, a generalist predator of brown planthopper Nilaparvata lugens.Scientific reports · 2016Article
- Ingestion of Bt corn pollen containing Cry1Ab/2Aj or Cry1Ac does not harm Propylea japonica larvae.Scientific reports · 2016Article
- The development and status of Bt rice in China.Plant biotechnology journal · 2016Review
- Field trials to evaluate the effects of transgenic cry1Ie maize on the community characteristics of arthropod natural enemies.Scientific reports · 2016Article
- Bt proteins Cry1Ah and Cry2Ab do not affect cotton aphid Aphis gossypii and ladybeetle Propylea japonica.Scientific reports · 2016Article
- Revised annual post-market environmental monitoring (PMEM) report on the cultivation of genetically modified maize MON 810 in 2013 from Monsanto Europe S.A.EFSA journal. European Food Safety Authority · 2015Article
- Toxicological and biochemical analyses demonstrate no toxic effect of Cry1C and Cry2A to Folsomia candida.Scientific reports · 2015Article
- Bt rice does not disrupt the host-searching behavior of the parasitoid Cotesia chilonis.Scientific reports · 2015Article
- Consumption of Bt rice pollen containing Cry1C or Cry2A does not pose a risk to Propylea japonica (Thunberg) (Coleoptera: Coccinellidae).Scientific reports · 2015Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rape seed pollen-based diet was developed and found to be suitable for use in a dietary exposure assay for Propylea japonica. Using the diet, we established and validated a dietary exposure assay by using the protease inhibitor E-64 as positive control. Dose-dependent responses were documented for all observed life-table parameters of P. japonica including survival, pupation and eclosion rates, development time and adult weight. Results suggested that the dietary assay can detect the effects of insecticidal compounds on the survival and development of P. japonica. Using the established dietary assay, we subsequently tested the toxicity of Cry1Ab, Cry1Ac and Cry1F proteins that are expressed by transgenic maize, cotton or rice plants to P. japonica larvae. The diet containing E-64 was included as a positive control. Survival and development of P. japonica larvae were not adversely affected when the diet contained purified Cry1Ab, Cry1Ac, or Cry1F at 500 µg/g diet representing a worst-case exposure scenario. In contrast, P. japonica larvae were adversely affected when the diet contained E-64. The bioactivity and stability of the Cry proteins in the diet and Cry protein uptake by the ladybird larvae were confirmed by bioassay with a Cry-sensitive insect species and by ELISA. The current study describes a suitable experimental system for assessing the potential effects of gut-active insecticidal compounds on ladybird beetle larvae. The experiments with the Cry proteins demonstrate that P. japonica larvae are not sensitive to Cry1Ab, Cry1Ac and Cry1F.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.