ArticlePloS one2014
Probing the subcellular localization of hopanoid lipids in bacteria using NanoSIMS.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Comparing Raman and NanoSIMS for heavy water labeling of single cells.Microbiology spectrum · 2025Article
- Bacterial lipid biophysics and membrane organization.Current opinion in microbiology · 2023Review
- Membrane composition and organization of Bacillus subtilis 168 and its genome-reduced derivative miniBacillus PG10.Microbial biotechnology · 2022Article
- The Genome Analysis of the Human Lung-AssociatedMicroorganisms · 2021Article
- The composition and function ofmicroLife · 2021Article
- A Novel Soluble Squalene-Hopene Cyclase and Its Application in Efficient Synthesis of Hopene.Frontiers in bioengineering and biotechnology · 2020Article
- Impact of Fatty-Acid Labeling ofFrontiers in microbiology · 2020Article
- Hopanoid lipids: from membranes to plant-bacteria interactions.Nature reviews. Microbiology · 2018Review
- Unraveling the role of membrane microdomains during microbial infections.Cell biology and toxicology · 2017Review
- Functional Membrane Microdomains Organize Signaling Networks in Bacteria.The Journal of membrane biology · 2017Review
- Lack of Methylated Hopanoids Renders the Cyanobacterium Nostoc punctiforme Sensitive to Osmotic and pH Stress.Applied and environmental microbiology · 2017Article
- Subcellular Chemical Imaging of Antibiotics in Single Bacteria Using CAnalytical chemistry · 2017Article
- Exploring functional membrane microdomains in bacteria: an overview.Current opinion in microbiology · 2017Review
- Global Fecal and Plasma Metabolic Dynamics Related toFrontiers in microbiology · 2017Article
- Hopanoid-free Methylobacterium extorquens DM4 overproduces carotenoids and has widespread growth impairment.PloS one · 2017Article
- Recent progress on lipid lateral heterogeneity in plasma membranes: From rafts to submicrometric domains.Progress in lipid research · 2016Review
- Focal Targeting of the Bacterial Envelope by Antimicrobial Peptides.Frontiers in cell and developmental biology · 2016Review
- Heavy water and (15) N labelling with NanoSIMS analysis reveals growth rate-dependent metabolic heterogeneity in chemostats.Environmental microbiology · 2015Article
- Quantitative hopanoid analysis enables robust pattern detection and comparison between laboratories.Geobiology · 2015Article
- Exploring the existence of lipid rafts in bacteria.Microbiology and molecular biology reviews : MMBR · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The organization of lipids within biological membranes is poorly understood. Some studies have suggested lipids group into microdomains within cells, but the evidence remains controversial due to non-native imaging techniques. A recently developed NanoSIMS technique indicated that sphingolipids group into microdomains within membranes of human fibroblast cells. We extended this NanoSIMS approach to study the localization of hopanoid lipids in bacterial cells by developing a stable isotope labeling method to directly detect subcellular localization of specific lipids in bacteria with ca. 60 nm resolution. Because of the relatively small size of bacterial cells and the relative abundance of hopanoid lipids in membranes, we employed a primary (2)H-label to maximize our limit of detection. This approach permitted the analysis of multiple stable isotope labels within the same sample, enabling visualization of subcellular lipid microdomains within different cell types using a secondary label to mark the growing end of the cell. Using this technique, we demonstrate subcellular localization of hopanoid lipids within alpha-proteobacterial and cyanobacterial cells. Further, we provide evidence of hopanoid lipid domains in between cells of the filamentous cyanobacterium Nostoc punctiforme. More broadly, our method provides a means to image lipid microdomains in a wide range of cell types and test hypotheses for their functions in membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.