ArticleThe Journal of cell biology1987
An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 341 citations in OpenAlex.
- Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.Plant cell reports · 2025Article
- Simulated actin reorganization mediated by motor proteins.PLoS computational biology · 2022Article
- A rich and bountiful harvest: Key discoveries in plant cell biology.The Plant cell · 2022Review
- Methods to Visualize the Actin Cytoskeleton During Plant Cell Division.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Plant cell divisions: variations from the shortest symmetric path.Biochemical Society transactions · 2020Review
- Cytoskeletal organization in isolated plant cells under geometry control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos.PLoS computational biology · 2019Article
- DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis.eLife · 2018Article
- Myosin XI motors: back on the scene at the division machine.Journal of experimental botany · 2018Article
- A toolkit for studying cellular reorganization during early embryogenesis in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2018Article
- Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A.Plant reproduction · 2017Article
- Single microfilaments mediate the early steps of microtubule bundling during preprophase band formation in onion cotyledon epidermal cells.Molecular biology of the cell · 2016Article
- Impact of Phenylpropanoid Compounds on Heat Stress Tolerance in Carrot Cell Cultures.Frontiers in plant science · 2016Article
- Multifunctional Microtubule-Associated Proteins in Plants.Frontiers in plant science · 2016Review
- Preprophase band formation and cortical division zone establishment: RanGAP behaves differently from microtubules during their band formation.Plant signaling & behavior · 2015Article
- Strategies of seedlings to overcome their sessile nature: auxin in mobility control.Frontiers in plant science · 2015Review
- Rhinanthus serotinus (Schönheit) Oborny (Scrophulariaceae): immunohistochemical and ultrastructural studies of endosperm chalazal haustorium development.Protoplasma · 2013Article
- The dual functions of WLIM1a in cell elongation and secondary wall formation in developing cotton fibers.The Plant cell · 2013Article
- Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform.PloS one · 2013Article
- Arabidopsis SOS3 plays an important role in salt tolerance by mediating calcium-dependent microfilament reorganization.Plant cell reports · 2013Article
5 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the F-actin network in cycling suspension culture cells of carrot (Daucus carota L.) using rhodaminyl lysine phallotoxin (RLP). In addition to conventional fixation with formaldehyde, we have used two different nonfixation methods before adding RLP: extracting cells in a stabilizing buffer; inducing transient pores in the plasma membrane with pulses of direct current (electroporation). These alternative methods for introducing RLP revealed additional features of the actin network not seen in aldehyde-fixed cells. The three-dimensional organization of this network in nonflattened cells was demonstrated by projecting stereopairs derived from through-focal series of computer-enhanced images. F-actin is present in interphase cells in four interconnected configurations: a meshwork surrounding the nucleus; thick cables in transvacuolar strands and deep in the cytoplasm; a finer network of bundles within the cortical cytoplasm; even finer filaments that run in ordered transverse array around the cell periphery. The actin network is organized differently during division but it does not disappear as do the cortical microtubules. RLP stains a central filamentous cortical band as the chromatin begins to condense (preprophase); it stains the mitotic spindle (as recently shown by Seagull et al. [Seagull, R. W., M. Falconer, and C. A. Weerdenburg, 1987, J. Cell Biol., 104:995-1004] for aldehyde fixed suspension cells) and the cytokinetic apparatus (as shown by Clayton, L., and C. W. Lloyd, 1985, Exp. Cell Res., 156:231-238). However, it is now shown that an additional network of F-actin persists in the cytoplasm throughout division associating in turn with the preprophase band, the mitotic spindle, and the cytokinetic phragmoplast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.