ArticleThe Journal of cell biology1987
Changes in villin synthesis and subcellular distribution during intestinal differentiation of HT29-18 clones.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 148 citations in OpenAlex.
- MTGR1 is required to maintain small intestinal stem cell populations.Cell death and differentiation · 2024Article
- Mitotic Spindle Positioning (MISP) is an actin bundler that selectively stabilizes the rootlets of epithelial microvilli.Cell reports · 2022Article
- Extracellular nucleotides inhibit oxalate transport by human intestinal Caco-2-BBe cells through PKC-δ activation.American journal of physiology. Cell physiology · 2013Article
- Article
- Actin reorganization as the molecular basis for the regulation of apoptosis in gastrointestinal epithelial cells.Cell death and differentiation · 2012Article
- Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells.American journal of physiology. Cell physiology · 2012Article
- Villin and actin in the mouse kidney brush-border membrane bind to and are degraded by meprins, an interaction that contributes to injury in ischemia-reperfusion.American journal of physiology. Renal physiology · 2011Article
- Regulation of villin by wnt5a/ror2 signaling in human intestinal cells.Frontiers in physiology · 2011Article
- Comparison of six immunohistochemical markers for the histologic diagnosis of neoplasia in Barrett's esophagus.Virchows Archiv : an international journal of pathology · 2010Article
- Schlafen 3 induction by cyclic strain regulates intestinal epithelial differentiation.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Small intestinal mucosa expression of putative chaperone fls485.BMC gastroenterology · 2010Article
- Regulation of cell structure and function by actin-binding proteins: villin's perspective.FEBS letters · 2008Review
- Antigen transport and cytoskeletal characteristics of a distinct enterocyte population in inflammatory bowel diseases.The American journal of pathology · 2004Article
- Villin enhances hepatocyte growth factor-induced actin cytoskeleton remodeling in epithelial cells.Molecular biology of the cell · 2003Article
- Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis.Plant physiology · 2000Article
- Differential effects of deoxycholic acid on proliferation of neoplastic and differentiated colonocytes in vitro.Digestive diseases and sciences · 1997Article
- Suppression of villin expression by antisense RNA impairs brush border assembly in polarized epithelial intestinal cells.The EMBO journal · 1995Article
- Regulation of CFTR expression and function during differentiation of intestinal epithelial cells.The EMBO journal · 1992Article
- Intestinal epithelial restitution. Characterization of a cell culture model and mapping of cytoskeletal elements in migrating cells.The Journal of clinical investigation · 1992Article
- In vivo analysis of functional domains from villin and gelsolin.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brush border in enterocytes is a cell surface specialization intimately associated with terminal differentiation of these cells. HT29-18, a clone derived from the HT-29 human colonic adenocarcinoma cell line, and HT29-18-C1, a subclone from HT29-18 described in the companion paper (Huet, C., C. Sahuquillo-Merino, E. Coudrier, and D. Louvard, 1987, J. Cell Biol., 105:345-357), undergo terminal differentiation with brush borders in the absence of glucose or upon replacement of glucose by galactose in the medium. Taking advantage of this clone and its subclone which can be manipulated in vitro, we have studied the synthesis and subcellular distribution of villin, one major protein in the microvillus core of the brush border. For this study, a monoclonal antibody against villin (BDID2C3) has been isolated and characterized in detail. In addition an ELISA has been set up to measure villin accurately in total cell extracts. Villin content in differentiated HT29-18 cells is close to that seen in normal human colonic cells but 10 times lower in undifferentiated HT29-18 cells. The rate of villin synthesis is dramatically increased in the course of enterocytic differentiation, while villin is remarkably stable after synthesis. We have recently shown, using a cDNA probe for villin, that this change is controlled either at the transcription level or by RNA stabilization (Pringault, E., M. Arpin, A. Garcia, J. Finidori, and D. Louvard, 1986, EMBO (Eur. Mol. Biol. Organ.) J., 5:3119-3124). As shown by immunofluorescence and immunogold labelings, villin is targeted to the brush border area of differentiated HT29-18 cells but remains diffusely distributed in undifferentiated ones.
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