Evidence map›Paper›PMID 24407592›Full record

ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2014

Amniotic fluid-borne hepatocyte growth factor protects rat pups against experimental necrotizing enterocolitis.

Sunil K Jain, Eric W Baggerman, Krishnan Mohankumar, Kopperuncholan Namachivayam, Ramasamy Jagadeeswaran, Victor E Reyes, Akhil Maheshwari

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Gastrointestinal and liver physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sunil K JainDepartment of Pediatrics (Division of Neonatology), University of Texas Medical Branch, Galveston, Texas;
Eric W Baggerman
Krishnan Mohankumar
Kopperuncholan Namachivayam
Ramasamy Jagadeeswaran
Victor E Reyes
Akhil Maheshwari
University of Illinois Chicago · USThe University of Texas Medical Branch at Galveston · US

Funding

Role of Transforming Growth Factor-beta in Neonatal Necrotizing EnterocolitisR01HD059142 · NICHD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MAHESHWARI, AKHIL · 2009 to 2013
$1.7M
NICHD NIH HHS R01 HD059142NICHD NIH HHS R01HD059142
6 · The paper itself

Abstract

Fetal swallowing of amniotic fluid, which contains numerous cytokines and growth factors, plays a key role in gut mucosal development. Preterm birth interrupts this exposure to amniotic fluid-borne growth factors, possibly contributing to the increased risk of necrotizing enterocolitis (NEC) in premature infants. We hypothesized that supplementation of formula feeds with amniotic fluid can provide amniotic fluid-borne growth factors and prevent experimental NEC in rat pups. We compared NEC-like injury in rat pups fed with infant formula vs. formula supplemented either with 30% amniotic fluid or recombinant hepatocyte growth factor (HGF). Cytokines/growth factors in amniotic fluid were measured by immunoassays. Amniotic fluid and HGF effects on enterocyte migration, proliferation, and survival were measured in cultured IEC6 intestinal epithelial cells. Finally, we used an antibody array to investigate receptor tyrosine kinase (RTK) activation and immunoblots to measure phosphoinositide 3-kinase (PI3K) signaling. Amniotic fluid supplementation in oral feeds protected rat pups against NEC-like injury. HGF was the most abundant growth factor in rat amniotic fluid in our panel of analytes. Amniotic fluid increased cell migration, proliferation, and cell survival in vitro. These effects were reproduced by HGF and blocked by anti-HGF antibody or a PI3K inhibitor. HGF transactivated several RTKs in IEC6 cells, indicating that its effects extended to multiple signaling pathways. Finally, similar to amniotic fluid, recombinant HGF also reduced the frequency and severity of NEC-like injury in rat pups. Amniotic fluid supplementation protects rat pups against experimental NEC, which is mediated, at least in part, by HGF.

Indexed as

Amniotic FluidAnimal FeedAnimalsCells, CulturedCytokinesDietary SupplementsEnterocolitis, NecrotizingEpithelial CellsFemaleGene Expression Regulation, EnzymologicHepatocyte Growth FactorHumansInfantInfant FormulaIntestinal MucosaPregnancyCytokinesHepatocyte Growth FactorReceptor Protein-Tyrosine Kinasesamniotic fluidHGFinflammationNECphosphoinositide 3-kinase

Identifiers

PMID24407592
PMCPMC3949017
OpenAlexW2101500188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.