ArticleClinical oral investigations2014
Gene expression profile of compressed primary human cementoblasts before and after IL-1β stimulation.
Article in Clinical oral investigations, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Effect of micro-osteoperforations on the gene expression profile of the periodontal ligament of orthodontically moved human teeth.Clinical oral investigations · 2022Trial
- Cell Death in Orthodontic Tooth Movement: Recent Advances and Emerging Insights.International journal of molecular sciences · 2026Review
- Ferroptosis inhibits cementoblast mineralization via cGAS-STING/GPX4 axis.Progress in orthodontics · 2026Article
- Mitochondrial ROS participates inHeliyon · 2024Article
- Compressive force strengthened the pro-inflammatory effect of zoledronic acid on il-1ß stimulated human periodontal fibroblasts.Clinical oral investigations · 2021Article
- Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.Clinical oral investigations · 2021Article
- Yes-associated protein promotes tumour necrosis factor α-treated cementoblast mineralization partly by inactivating NF-κB pathway.Journal of cellular and molecular medicine · 2020Article
- Influence of clodronate and compressive force on IL-1ß-stimulated human periodontal ligament fibroblasts.Clinical oral investigations · 2020Article
- Effect of caspases and RANKL induced by heavy force in orthodontic root resorption.Korean journal of orthodontics · 2018Article
- Inhibition of AXUD1 attenuates compression-dependent apoptosis of cementoblasts.Clinical oral investigations · 2016Article
- Compression of human primary cementoblasts leads to apoptosis: A possible cause of dental root resorption?Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesRoot resorptions due to a reduced repair function of cementoblasts are common side effects during orthodontic tooth movement (OTM). The mechanisms, which lead to an impaired cementoblast function, are not fully understood. Therefore, we aimed to investigate changes in the gene expression of cementoblasts during mechanical stimulus under inflammatory conditions. MATERIALS AND
methodsHuman primary cementoblasts (HPCB) were exposed to compression for 6 h or stimulation with IL-1β for 96 h and subsequent 6 h compression. Genome-wide expression analysis was performed using microarray analysis. Prominent gene expression alterations (COX2, AXUD1, FOSB, CCL2, IFI6, and PTGES) were verified by quantitative RT-PCR (qRT-PCR) in two HPCB populations. A caspase 3/7 activity assay was used to determine caspase-3 and caspase-7 activity in stressed cells.
resultsGene expression cluster analysis revealed apoptosis as an important process induced under both conditions. Apoptosis (pro- and anti-apoptotic) related gene expression was most relevant after pro-inflammatory stimulation and compression. qRT-PCR analysis confirmed significant up-regulation of COX2, AXUD1, and FOSB in both HPCB populations after compression, while selected genes significantly increased after pro-inflammatory stimulation and compression. Compression of cementoblasts increased caspase. The combination of pro-inflammatory stimulation and compression led to a slightly smaller increase of caspase activity.
conclusionsGene ontology analysis showed that compressed HPCB up-regulate genes that are associated with apoptosis. Combining compression with a pro-inflammatory stimulus (IL-1β) augmented the positive regulation of apoptosis-related pathways. The induction of apoptosis related gene expression (pro- and anti-apoptotic genes) in cementoblasts suggests an involvement of apoptosis in cementoblast regulation during OTM. CLINICAL RELEVANCE: As apoptosis is induced in HPCB after compression and inflammation, it is conceivable that HPCB cell death might contribute to root resorptions due to a loss of repair activity of cementoblasts. Further studies should be conducted to clarify the implication of the identified genes on root resorptions in order to develop therapeutic strategies to prevent a shortening of roots.
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