ArticlePlant physiology2014
Lignin modification leads to increased nodule numbers in alfalfa.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Unearthing Root Response Mechanisms to Soil Compaction in Legumes.Plant, cell & environment · 2026Review
- Transcriptomic Comparison of Soybean Roots Inoculated with Different Rhizobium Strains During Early Symbiosis.Plants (Basel, Switzerland) · 2026Article
- Advances in the Function Roles of Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferases: A Key Enzyme Linking Phenylpropanoid Metabolism to Plant Terrestrial Adaptation.Plants (Basel, Switzerland) · 2026Review
- Effect of Insecticides Imidacloprid and Alpha-Cypermethrin on the Development of Pea (Plants (Basel, Switzerland) · 2024Article
- Alfalfa Responses to Intensive Soil Compaction: Effects on Plant and Root Growth, Phytohormones and Internal Gene Expression.Plants (Basel, Switzerland) · 2024Article
- Mutating alfalfaFrontiers in plant science · 2024Article
- A comprehensive review on genetic modification of plant cell wall for improved saccharification efficiency.Molecular biology reports · 2023Review
- Comprehensive physiological, transcriptomic, and metabolomic analyses reveal the synergistic mechanism ofFrontiers in plant science · 2022Article
- Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production.Journal of advanced research · 2020Review
- APETALA 2 transcription factor CBX1 is a regulator of mycorrhizal symbiosis and growth of Lotus japonicus.Plant cell reports · 2020Article
- Comparative genomics of the nonlegumeProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture.International journal of environmental research and public health · 2018Review
- Distinctive Patterns of Flavonoid Biosynthesis in Roots and Nodules ofFrontiers in plant science · 2018Article
- MsmiR156 affects global gene expression and promotes root regenerative capacity and nitrogen fixation activity in alfalfa.Transgenic research · 2017Article
- Multigene synergism increases the isoflavone and proanthocyanidin contents of Medicago truncatula.Plant biotechnology journal · 2016Article
- Characterization of the plant growth promoting bacterium, Enterobacter cloacae MSR1, isolated from roots of non-nodulating Medicago sativa.Saudi journal of biological sciences · 2016Article
- Unity in diversity, a systems approach to regulating plant cell physiology by 2-oxoglutarate-dependent dioxygenases.Frontiers in plant science · 2015Article
- Phytohormone regulation of legume-rhizobia interactions.Journal of chemical ecology · 2014Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reduction of lignin levels in the forage legume alfalfa (Medicago sativa) by down-regulation of the monolignol biosynthetic enzyme hydroxycinnamoyl coenzyme A:shikimate hydroxycinnamoyl transferase (HCT) results in strongly increased digestibility and processing ability of lignocellulose. However, these modifications are often also associated with dwarfing and other changes in plant growth. Given the importance of nitrogen fixation for legume growth, we evaluated the impact of constitutively targeted lignin modification on the belowground organs (roots and nodules) of alfalfa plants. HCT down-regulated alfalfa plants exhibit a striking reduction in root growth accompanied by an unexpected increase in nodule numbers when grown in the greenhouse or in the field. This phenotype is associated with increased levels of gibberellins and certain flavonoid compounds in roots. Although HCT down-regulation reduced biomass yields in both the greenhouse and field experiments, the impact on the allocation of nitrogen to shoots or roots was minimal. It is unlikely, therefore, that the altered growth phenotype of reduced-lignin alfalfa is a direct result of changes in nodulation or nitrogen fixation efficiency. Furthermore, HCT down-regulation has no measurable effect on carbon allocation to roots in either greenhouse or 3-year field trials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.