ArticleReproductive sciences (Thousand Oaks, Calif.)2014
Abnormal DNA Methylation of Imprinted Loci in Human Preimplantation Embryos.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Blastocyst culture of non-top-quality cleavage embryos may increase the risk of anembryonic pregnancy followingAnnals of translational medicine · 2022Article
- DNA Methylation in Offspring Conceived after Assisted Reproductive Techniques: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2022Review
- Review
- Association of four imprinting disorders and ART.Clinical epigenetics · 2019Article
- ART manipulation after controlled ovarian stimulation may not increase the risk of abnormal expression and DNA methylation at some CpG sites of H19,IGF2 and SNRPN in foetuses: a pilot study.Reproductive biology and endocrinology : RB&E · 2018Article
- Mosaicism for GNAS methylation defects associated with pseudohypoparathyroidism type 1B arose in early post-zygotic phases.Clinical epigenetics · 2018Article
- DNA methyltransferase 3A promoter polymorphism is associated with the risk of human spontaneous abortion after assisted reproduction techniques and natural conception.Journal of assisted reproduction and genetics · 2017Article
- iTRAQ-based quantitative proteomic analysis reveals the distinct early embryo myofiber type characteristics involved in landrace and miniature pig.BMC genomics · 2016Article
- Epigenetic Dysregulation Observed in Monosomy Blastocysts Further Compromises Developmental Potential.PloS one · 2016Article
- High Frequency of Imprinted Methylation Errors in Human Preimplantation Embryos.Scientific reports · 2015Article
- Genes and Conditions Controlling Mammalian Pre- and Post-implantation Embryo Development.Current genomics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the epigenetic risk linked to assisted reproductive technology (ART) at single-embryo level by analyzing the methylation status of imprinted H19, PEG1, and KvDMR1 in human preimplantation embryos.
methodsA total of 254 human day 3 embryos produced by ART procedures were collected. All embryos were normally fertilized but unsuitable for transfer or freezing due to poor quality. Pyrosequencing with confirmatory routine bisulfite sequencing were used to determine the DNA methylation patterns of H19 differentially methylated region (DMR) in 63 embryos, PEG1 DMR in 65 embryos, and KvDMR1 in 67 embryos.
resultsAberrant methylation patterns were found in 8.0% embryos at H19 DMR, 16.9% embryos at PEG1 DMR, and 10.4% embryos at KvDMR1. No methylation errors were found in corresponding sperm samples.
conclusionsWe hypothesized that the use of poor-quality embryos may increase the risk of imprinting defects because they might have methylation errors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.