Evidence map›Paper›PMID 24406728›Full record

ArticleInternational journal of molecular sciences2014

An improved MLVF method and its comparison with traditional MLVF, spa typing, MLST/SCCmec and PFGE for the typing of methicillin-resistant Staphylococcus aureus.

Xue-Fei Du, Meng Xiao, Hong-Yan Liang, Zhe Sun, Yue-Hong Jiang, Guo-Yu Chen, Xiao-Yu Meng, Gui-Ling Zou, Li Zhang, Ya-Li Liu and 4 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Molecular Epidemiology ofAntibiotics (Basel, Switzerland) · 2020
    Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xue-Fei DuDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. duxuefei82@163.com.
Meng XiaoDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. xmpumch@126.com.
Hong-Yan LiangDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. lhyhydsy@126.com.
Zhe SunDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. sunsz00000@163.com.
Yue-Hong JiangDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. jiangyuehonghydsy@126.com.
Guo-Yu ChenDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. cgyhydsy@126.com.
Xiao-Yu MengDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. mxyhydsy@126.com.
Gui-Ling ZouDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. zglhydsy@126.com.
Li ZhangDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. zlpumch@126.com.
Ya-Li LiuDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. lylpumch@126.com.
Hui ZhangDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. zhpumch@126.com.
Hong-Li SunDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. shlpumch@126.com.
Xiao-Feng JiangDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. jiangxiaofeng12359@126.com.
Ying-Chun XuDepartment of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China. xycpumch@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has become an important nosocomial pathogen, causing considerable morbidity and mortality. During the last 20 years, a variety of genotyping methods have been introduced for screening the prevalence of MRSA. In this study, we developed and evaluated an improved approach capillary gel electrophoresis based multilocus variable-number tandem-repeat fingerprinting (CGE/MLVF) for rapid MRSA typing. A total of 42 well-characterized strains and 116 non-repetitive clinical MRSA isolates collected from six hospitals in northeast China between 2009 and 2010 were tested. The results obtained by CGE/MLVF against clinical isolates were compared with traditional MLVF, spa typing, Multilocus sequence typing/ staphylococcal cassette chromosome mec (MLST/SCCmec) and pulse field gel electrophoresis (PFGE). The discriminatory power estimated by Simpson's index of diversity was 0.855 (28 types), 0.855 (28 patterns), 0.623 (11 types), 0.517 (8 types) and 0.854 (28 patterns) for CGE/MLVF, traditional MLVF, spa typing, MLST/SCCmec and PFGE, respectively. All methods tested showed a satisfied concordance in clonal complex level calculated by adjusted Rand's coefficient. CGE/MLVF showed better reproducibility and accuracy than traditional MLVF and PFGE methods. In addition, the CGE/MLVF has potential to produce portable results. In conclusion, CGE/MLVF is a rapid and easy to use MRSA typing method with lower cost, good reproducibility and high discriminatory power for monitoring the outbreak and clonal spread of MRSA isolates.

Indexed as

Multiplex Polymerase Chain ReactionDNA, BacterialDNA FingerprintingElectrophoresis, CapillaryElectrophoresis, Gel, Pulsed-FieldMethicillin-Resistant Staphylococcus aureusMultilocus Sequence TypingStaphylococcal Protein ATandem Repeat SequencesDNA, BacterialStaphylococcal Protein A

Identifiers

PMID24406728
PMCPMC3907834

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.