ArticleBioengineered
Whole cell biotransformation for reductive amination reactions.
Article in Bioengineered. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Compositional changes and ecological characteristics of earthworm mucus under different electrical stimuli.Scientific reports · 2023Article
- Construction of a Self-Purification and Self-Assembly Coenzyme Regeneration System for the Synthesis of Chiral Drug Intermediates.ACS omega · 2021Article
- A Wake-Up Call for the Efficient Use of the Bacterial Resting Cell Process, with Focus on Low Solubility Products.Current microbiology · 2020Review
- Efficient synthesis of enantiopure amines from alcohols using restingGreen chemistry : an international journal and green chemistry resource : GC · 2019Article
- Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural.Applied and environmental microbiology · 2017Article
- Construction of a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates.Scientific reports · 2016Article
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3 authors.
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Abstract
Whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. Recently, we established an Escherichia coli whole cell biotransformation system for reductive amination by coupling a transaminase and an amino acid dehydrogenase with glucose catabolism for cofactor recycling. Transformation of 2-keto-3-methylvalerate to l-isoleucine by E. coli cells was improved by genetic engineering of glucose metabolism for improved cofactor regeneration. Here, we compare this system with different strategies for cofactor regeneration such as cascading with alcohol dehydrogenases, with alternative production hosts such as Pseudomonas species or Corynebacterium glutamicum, and with improving whole cell biotransformation systems by metabolic engineering of NADPH regeneration.
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