Observational studyBMC infectious diseases2014
Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control.
Observational study in BMC infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- The Respiratory Specimen Collection Trial (ReSpeCT): A Randomized Controlled Trial to Compare Quality and Timeliness of Respiratory Sample Collection in the Home by Parents and Healthcare Workers From Children Aged <2 Years.Journal of the Pediatric Infectious Diseases Society · 2020Trial
- Comparing Polymerase Chain Reaction Testing of Nasopharyngeal Swab and Lower Respiratory Tract Specimens for the Diagnosis ofOpen forum infectious diseases · 2024Article
- Human bocavirus-1 infections in Australian children aged < 2 years: a birth cohort study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Exposure to bacterial and fungal bioaerosols in facilities processing biodegradable waste.Frontiers in public health · 2022Article
- Prospective Study of the Performance of Parent-Collected Nasal and Saliva Swab Samples, Compared with Nurse-Collected Swab Samples, for the Molecular Detection of Respiratory Microorganisms.Microbiology spectrum · 2021Observational
- Upper Respiratory Tract Microbiome of Australian Aboriginal and Torres Strait Islander Children in Ear and Nose Health and Disease.Microbiology spectrum · 2021Article
- Epidemiology of respiratory syncytial virus in a community birth cohort of infants in the first 2 years of life.European journal of pediatrics · 2021Article
- Over 90% of clinical swabs used for SARS-CoV-2 diagnostics contain sufficient nucleic acid concentrations.Journal of medical virology · 2021Article
- Time variation in the probability of failing to detect a case of polymerase chain reaction testing for SARS-CoV-2 as estimated from a viral dynamics model.Journal of the Royal Society, Interface · 2021Article
- The role of rhinovirus infections in young children with cystic fibrosis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Nasal swab bacteriology by PCR during the first 24-months of life: A prospective birth cohort study.Pediatric pulmonology · 2019Observational
- Viruses causing lower respiratory symptoms in young children: findings from the ORChID birth cohort.Thorax · 2018Observational
- Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- Timing of First Respiratory Virus Detections in Infants: A Community-Based Birth Cohort Study.The Journal of infectious diseases · 2018Article
- The association between anterior nares and nasopharyngeal microbiota in infants hospitalized for bronchiolitis.Microbiome · 2018Article
- Comparative Viral Sampling in the Sinonasal Passages; Different Viruses at Different Sites.Frontiers in cellular and infection microbiology · 2018Article
- Acute viral respiratory infections among children in MERS-endemic Riyadh, Saudi Arabia, 2012-2013.Journal of medical virology · 2017Article
- Endogenous Retrovirus 3 - History, Physiology, and Pathology.Frontiers in microbiology · 2017Review
- Study and interest of cellular load in respiratory samples for the optimization of molecular virological diagnosis in clinical practice.BMC infectious diseases · 2016Article
- Pilot study of participant-collected nasal swabs for acute respiratory infections in a low-income, urban population.Clinical epidemiology · 2016Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCarefully conducted, community-based, longitudinal studies are required to gain further understanding of the nature and timing of respiratory viruses causing infections in the population. However, such studies pose unique challenges for field specimen collection, including as we have observed the appearance of mould in some nasal swab specimens. We therefore investigated the impact of sample collection quality and the presence of visible mould in samples upon respiratory virus detection by real-time polymerase chain reaction (PCR) assays.
methodsAnterior nasal swab samples were collected from infants participating in an ongoing community-based, longitudinal, dynamic birth cohort study. The samples were first collected from each infant shortly after birth and weekly thereafter. They were then mailed to the laboratory where they were catalogued, stored at -80°C and later screened by PCR for 17 respiratory viruses. The quality of specimen collection was assessed by screening for human deoxyribonucleic acid (DNA) using endogenous retrovirus 3 (ERV3). The impact of ERV3 load upon respiratory virus detection and the impact of visible mould observed in a subset of swabs reaching the laboratory upon both ERV3 loads and respiratory virus detection was determined.
resultsIn total, 4933 nasal swabs were received in the laboratory. ERV3 load in nasal swabs was associated with respiratory virus detection. Reduced respiratory virus detection (odds ratio 0.35; 95% confidence interval 0.27-0.44) was observed in samples where the ERV3 could not be identified. Mould was associated with increased time of samples reaching the laboratory and reduced ERV3 loads and respiratory virus detection.
conclusionSuboptimal sample collection and high levels of visible mould can impact negatively upon sample quality. Quality control measures, including monitoring human DNA loads using ERV3 as a marker for epithelial cell components in samples should be undertaken to optimize the validity of real-time PCR results for respiratory virus investigations in community-based studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.