ArticleStem cell research & therapy2013
Molecular characterization and in vitro differentiation of feline progenitor-like amniotic epithelial cells.
Article in Stem cell research & therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- Isolation and characterization of feline dental pulp stem cells.Journal of feline medicine and surgery · 2023Article
- Feline hypertrophic cardiomyopathy: reduced microvascular density and involvement of CD34+ interstitial cells.Veterinary pathology · 2022Article
- Viability, yield and expansion capability of feline MSCs obtained from subcutaneous and reproductive organ adipose depots.BMC veterinary research · 2021Article
- Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications.International journal of molecular sciences · 2020Review
- Characterization of mesenchymal stem cells derived from adipose tissue of a cougar (Animal reproduction · 2020Article
- Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells.Stem cells and cloning : advances and applications · 2020Article
- Spare Parts from Discarded Materials: Fetal Annexes in Regenerative Medicine.International journal of molecular sciences · 2019Review
- Prostaglandin EBMC veterinary research · 2018Article
- Article
- Article
- Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells.Journal of veterinary science · 2017Article
- Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells.Stem cell research & therapy · 2017Article
- Amniotic membrane mesenchymal stem cells can differentiate into germ cells in vitro.In vitro cellular & developmental biology. Animal · 2016Article
- Human amniotic epithelial cells combined with silk fibroin scaffold in the repair of spinal cord injury.Neural regeneration research · 2016Article
- Article
- Dasatinib enhances tumor growth in gemcitabine-resistant orthotopic bladder cancer xenografts.BMC research notes · 2016Article
- Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis.Stem cell research & therapy · 2015Article
- Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro.In vitro cellular & developmental biology. Animal · 2015Article
- Drug-Resistant Urothelial Cancer Cell Lines Display Diverse Sensitivity Profiles to Potential Second-Line Therapeutics.Translational oncology · 2015Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionWhile amniotic mesenchymal cells have been isolated and characterized in different species, amniotic epithelial cells (AECs) have been found only in humans and horses and are recently considered valid candidates in regenerative medicine. The aim of this work is to obtain and characterize, for the first time in the feline species, presumptive stem cells from the epithelial portion of the amnion (AECs) to be used for clinical applications.
methodsIn our study, we molecularly characterized and induced in vitro differentiation of feline AECs, obtained after enzymatic digestion of amnion.
resultsAECs displayed a polygonal morphology and the mean doubling time value was 1.94 ± 0.04 days demonstrating the high proliferating capacity of these cells. By RT-PCR, AECs expressed pluripotent (Oct4, Nanog) and some mesenchymal markers (CD166, CD44) suggesting that an epithelial-mesenchymal transition may occur in these cells that lack the hematopoietic marker CD34. Cells also showed the expression of embryonic marker SSEA-4, but not SSEA-3, as demonstrated by immunocytochemistry and flow cytometry. Moreover, the possibility to use feline AECs in cell therapies resides in their low immunogenicity, due to the absence of MHC-II antigen expression. After induction, AECs differentiated into the mesodermic and ectodermic lineages, demonstrating high plasticity.
conclusionsIn conclusion, feline AECs appear to be a readily obtainable, highly proliferative, multipotent and non-immunogenic cell line from a source that may represent a good model system for stem cell biology and be useful in allogenic cell-based therapies in order to treat tissue lesions, especially with loss of substance.
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