ArticleBiomicrofluidics2013
Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore.
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Protein-free membrane fusion: A refined view of the delicate fusogenic properties of calcium.Biophysical journal · 2026Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Microrail-assisted liposome trapping and aligning in microfluidic channels.RSC advances · 2024Article
- Efficient liposome fusion to phase-separated giant vesicles.Biophysical journal · 2023Article
- Compartmentalized Cell-Free Expression Systems for Building Synthetic Cells.Advances in biochemical engineering/biotechnology · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles.Soft matter · 2022Article
- Article
- Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides.Analytical chemistry · 2022Article
- Identifying and Manipulating Giant Vesicles: Review of Recent Approaches.Micromachines · 2022Review
- Automated optimization of endoderm differentiation on chip.Lab on a chip · 2021Article
- Droplet Microfluidics for Food and Nutrition Applications.Micromachines · 2021Review
- Standardizing characterization of membrane active peptides with microfluidics.Biomicrofluidics · 2021Article
- Permeation Studies across Symmetric and Asymmetric Membranes in Microdroplet Arrays.Analytical chemistry · 2021Article
- Giant Vesicles Produced with Phosphatidylcholines (PCs) and Phosphatidylethanolamines (PEs) by Water-in-Oil Inverted Emulsions.Life (Basel, Switzerland) · 2021Article
- Sequential storage and release of microdroplets.Microsystems & nanoengineering · 2021Article
- FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps*.Angewandte Chemie (International ed. in English) · 2020Article
- Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes.Membranes · 2020Article
- Perfusion Chamber for Observing a Liposome-Based Cell Model Prepared by a Water-in-Oil Emulsion Transfer Method.ACS omega · 2020Article
- On-Chip Inverted Emulsion Method for Fast Giant Vesicle Production, Handling, and Analysis.Micromachines · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a microfluidic platform able to trap single GUVs in parallel. GUVs are used as model membranes across many fields of biophysics including lipid rafts, membrane fusion, and nanotubes. While their creation is relatively facile, handling and addressing single vesicles remains challenging. The PDMS microchip used herein contains 60 chambers, each with posts able to passively capture single GUVs without compromising their integrity. The design allows for circular valves to be lowered from the channel ceiling to isolate the vesicles from rest of the channel network. GUVs containing calcein were trapped and by rapidly opening the valves, the membrane pore protein α-hemolysin (αHL) was introduced to the membrane. Confocal microscopy revealed the kinetics of the small molecule efflux for different protein concentrations. This microfluidic approach greatly improves the number of experiments possible and can be applied to a wide range of biophysical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.