ArticleBiomicrofluidics2013
Protein sensing by nanofluidic crystal and its signal enhancement.
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Article
- Nanofluidic crystals: nanofluidics in a close-packed nanoparticle array.Lab on a chip · 2017Review
- Low auto-fluorescence fabrication methods for plastic nanoslits.IET nanobiotechnology · 2016Article
- A novel 2D silicon nano-mold fabrication technique for linear nanochannels over a 4 inch diameter substrate.Scientific reports · 2016Article
- Fabrication of two dimensional polyethylene terephthalate nanofluidic chip using hot embossing and thermal bonding technique.Biomicrofluidics · 2014Article
- Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor.Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
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Abstract
Nanofluidics has a unique property that ionic conductance across a nanometer-sized confined space is strongly affected by the space surface charge density, which can be utilized to construct electrical read-out biosensor. Based on this principle, this work demonstrated a novel protein sensor along with a sandwich signal enhancement approach. Nanoparticles with designed aptamer onside are assembled in a suspended micropore to form a 3-dimensional network of nanometer-sized interstices, named as nanofluidic crystal hereafter, as the basic sensing unit. Proteins captured by aptamers will change the surface charge density of nanoparticles and thereby can be detected by monitoring the ionic conductance across this nanofluidic crystal. Another aptamer can further enlarge the variations of the surface charge density by forming a sandwich structure (capturing aptamer/protein/signal enhancement aptamer) and the read-out conductance as well. The preliminary experimental results indicated that human α-thrombin was successfully detected by the corresponding aptamer modified nanofluidic crystal with the limit of detection of 5 nM (0.18 μg/ml) and the read-out signal was enhanced up to 3 folds by using another thrombin aptamer. Being easy to graft probe, facile and low-cost to prepare the nano-device, and having an electrical read-out, the present nanofluidic crystal scheme is a promising and universal strategy for protein sensing.
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Registered trials
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