ArticleBiomicrofluidics2013
A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay.
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- The Assessment of the Readiness of Molecular Biomarker-Based Mobile Health Technologies for Healthcare Applications.Scientific reports · 2015Pooled it
- Review
- 3D Printing for Cancer Diagnosis: What Unique Advantages Are Gained?ACS materials Au · 2023Review
- Shedding Light on Bladder Cancer Diagnosis in Urine.Diagnostics (Basel, Switzerland) · 2020Review
- Anti-p53 Autoantibody Detection in Automatic Glass Capillary Immunoassay Platform for Screening of Oral Cavity Squamous Cell Carcinoma.Sensors (Basel, Switzerland) · 2020Article
- Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.Technology · 2018Article
- Organ-Tumor-on-a-Chip for Chemosensitivity Assay: A Critical Review.Micromachines · 2016Review
- Novel developments in mobile sensing based on the integration of microfluidic devices and smartphones.Lab on a chip · 2016Review
- A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay.Biomicrofluidics · 2016Article
- Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping.Biomicrofluidics · 2015Article
- Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA.Scientific reports · 2015Article
- On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump.Biomicrofluidics · 2015Article
- Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay.Biomicrofluidics · 2014Article
- Direct detection of cancer biomarkers in blood using a "place n play" modular polydimethylsiloxane pump.Biomicrofluidics · 2013Article
- On-chip magnetophoretic isolation of CD4 + T cells from blood.Biomicrofluidics · 2013Article
- Microfluidic immunomagnetic cell separation using integrated permanent micromagnets.Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro-mixer, were driven by negative pressure, which simplifies the use of the chip and facilitates commercialization. Magnetic beads were used as a solid support for the primary antibody, which captured apolipoprotein A1 in patients' urine. Because of the three-dimensional structure of the magnetic beads, the concentration range of the target that could be detected was as high as 2000 ng ml(-1). Because this concentration is 100 times higher than that quantifiable using a 96-well plate with the same enzyme-linked immunosorbent assay (ELISA) kit, the dilution of the patient's urine can be avoided or greatly reduced. The limit of detection was determined to be approximately 10 ng ml(-1), which is lower than the cutoff value for diagnosing bladder cancer (11.16 ng ml(-1)). When the values measured using the microfluidic chip were compared with those measured using conventional ELISA using a 96-well plate for five patients, the deviations were 0.9%, 6.8%, 9.4%, 1.8%, and 5.8%. The entire measurement time is 6-fold faster than that of conventional ELISA. This microfluidic device shows significant potential for point-of-care applications.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.