ArticleBiomicrofluidics2013
Differential electronic detector to monitor apoptosis using dielectrophoresis-induced translation of flowing cells (dielectrophoresis cytometry).
Article in Biomicrofluidics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Light-Emitting Diode Array with Optical Linear Detector Enables High-Throughput Differential Single-Cell Dielectrophoretic Analysis.Sensors (Basel, Switzerland) · 2024Article
- On-chip dielectrophoretic single-cell manipulation.Microsystems & nanoengineering · 2024Review
- Apoptotic Bodies in the Pancreatic Tumor Cell Culture Media Enable Label-Free Drug Sensitivity Assessment by Impedance Cytometry.Advanced biology · 2021Article
- Dynamically controlled dielectrophoresis using resonant tuning.Electrophoresis · 2021Article
- Article
- Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells.Scientific reports · 2018Article
- Dielectrophoresis study of temporal change in internal conductivity of single CHO cells after electroporation by pulsed electric fields.Biomicrofluidics · 2017Article
- Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry.Biomicrofluidics · 2016Article
- Effect of a dual inlet channel on cell loading in microfluidics.Biomicrofluidics · 2014Article
- Monitoring the dielectric response of single cells following mitochondrial adenosine triphosphate synthase inhibition by oligomycin using a dielectrophoretic cytometer.Biomicrofluidics · 2014Article
- On-chip microelectrode impedance analysis of mammalian cell viability during biomanufacturing.Biomicrofluidics · 2014Article
- Microarray dot electrodes utilizing dielectrophoresis for cell characterization.Sensors (Basel, Switzerland) · 2013Article
- Sensitivity of bulk electrical impedance spectroscopy (bio-capacitance) probes to cell and culture properties: Study on CHO cell cultures.Biotechnology progressArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The instrument described here is an all-electronic dielectrophoresis (DEP) cytometer sensitive to changes in polarizability of single cells. The important novel feature of this work is the differential electrode array that allows independent detection and actuation of single cells within a short section ([Formula: see text]) of the microfluidic channel. DEP actuation modifies the altitude of the cells flowing between two altitude detection sites in proportion to cell polarizability; changes in altitude smaller than 0.25 μm can be detected electronically. Analysis of individual experimental signatures allows us to make a simple connection between the Clausius-Mossotti factor (CMF) and the amount of vertical cell deflection during actuation. This results in an all-electronic, label-free differential detector that monitors changes in physiological properties of the living cells and can be fully automated and miniaturized in order to be used in various online and offline probes and point-of-care medical applications. High sensitivity of the DEP cytometer facilitates observations of delicate changes in cell polarization that occur at the onset of apoptosis. We illustrate the application of this concept on a population of Chinese hamster ovary (CHO) cells that were followed in their rapid transition from a healthy viable to an early apoptotic state. DEP cytometer viability estimates closely match an Annexin V assay (an early apoptosis marker) on the same population of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.